Dynamics of the diversity of fungal and Fusarium communities during continuous cropping of cucumber in the greenhouse.
Dynamics of the diversity of fungal and Fusarium communities during continuous cropping of cucumber in the greenhouse.
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DOI：
10.1111/j.1574-6941.2012.01312.x
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发表时间：
2012-02
影响因子：
4.2
通讯作者：
Xingang Zhou;Fengzhi Wu
中科院分区：
文献类型：
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作者：
Xingang Zhou;Fengzhi Wu
黄瓜在同一盆土中连续种植可能导致作物产量和质量的降低，这种现象被描述为土壤病。连作对土壤微生物群落的影响及其与黄瓜土病的关系尚不清楚。在本研究中，黄瓜在温室条件下连续9个种植周期的盆栽。采用PCR-变性梯度凝胶电泳和定量逆转录PCR技术，对土壤中普遍存在的具有重要生态意义的真菌和镰刀菌（Ascomycota，Fungi）群落的结构和大小进行了分析。黄瓜在第七个种植周期表现出生长迟缓。同一土壤样品的RNA和DNA真菌群落结构存在差异，活性土壤真菌群落对连作更敏感。基于RNA的真菌和镰刀菌群落规模在第七个种植周期比其他种植周期更大。总的来说，这项研究的结果表明，人口规模，而不是多样性的真菌和镰刀菌群落与土壤病与黄瓜栽培。
The continuous cropping of cucumber in the same potting soils may result in a reduction of yield and quality of the crop, a phenomenon described as soil sickness. The changes of soil microbial communities as affected by continuous cropping and the link between these changes and soil sickness of cucumber are still not clear. In the present study, cucumber was cropped in pots under greenhouse conditions for nine successive cropping cycles. Structures and sizes of rhizosphere fungal and Fusarium (Ascomycota, Fungi) communities, both ubiquitous and ecologically important in soils, were analysed with PCR-denaturing gradient gel electrophoresis and quantitative reverse transcription PCR, respectively. Cucumber showed retarded growth in the seventh cropping cycle. The RNA- and DNA-based fungal community structures derived from the same sample differed from each other, and the active soil fungal communities were more sensitive to continuous cropping. The RNA-based fungal and Fusarium community sizes were larger in the seventh cropping cycle than in the other cropping cycles. Overall, the findings of this study indicate that the population sizes rather than the diversity of fungi and Fusarium communities are linked to the soil sickness associated with cucumber cultivation.