Abnormal expression of tyrosine hydroxylase immunoreactivity in cerebellar cortex of ataxic mutant mice
Abnormal expression of tyrosine hydroxylase immunoreactivity in cerebellar cortex of ataxic mutant mice
复制标题
共济失调突变小鼠小脑皮质酪氨酸羟化酶免疫反应性异常表达
DOI：
10.1016/s0006-8993(99)01347-5
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发表时间：
1998
期刊：
影响因子：
2.9
通讯作者：
Y. Fukui
中科院分区：
文献类型：
--
作者：
K. Sawada;S. Komatsu;H. Haga;Xuezhi Sun;S. Hisano;Y. Fukui
用免疫组织化学方法检测了两种共济失调突变小鼠名古屋（RMN）和稀释致死小鼠（DL）小脑中酪氨酸羟化酶（TH）的表达。在这两种突变体的同窝对照中，少数TH阳性浦肯野细胞稀疏分布，其数量小于在任何年龄检查的突变体。在RMN中，TH阳性浦肯野细胞分布于第IX小叶和第X小叶，并在2周龄时排列成旁带状。TH阳性浦肯野细胞的数量增加，并广泛分布在整个蚓部在3周龄。在成人RMN，TH阳性浦肯野细胞被发现在小脑的所有小叶。它们的副视带在半球也变得明显。在DL中，TH阳性浦肯野细胞主要分布在第IX和X小叶以及3周龄的绒球。在第IX小叶和第X小叶中也发现了这些条带。结果表明，TH在浦肯野细胞中的异常表达可能不是特异性的等位基因组。由于TH启动子被Ca ~（2+）激活，突变型浦肯野细胞中TH的表达可能预示细胞Ca ~（2+）电流改变引起的神经元功能障碍。
Expression of tyrosine hydroxylase (TH) was examined immunohistochemically in the cerebellum of two ataxic mutants, Rolling mouse Nagoya (RMN) and dilute-lethal mice (DL). In littermate controls of both mutants, a few TH-positive Purkinje cells were distributed sparsely and their number was smaller than in the mutants at any ages examined. In RMN, TH-positive Purkinje cells were distributed in lobule IX and X, and were arranged into parasagittal bands at 2 weeks of age. TH-positive Purkinje cells increased in number and were widely distributed throughout the vermis at 3 weeks of age. In adult RMN, TH-positive Purkinje cells were found in all lobules of the cerebellum. Their parasagittal bands also became evident in the hemisphere. In DL, TH-positive Purkinje cells were mainly distributed in vermal lobules IX and X, and the flocculus at 3 weeks of age. They were also found as bands in lobules IX and X. The results suggest that abnormal expression of TH in Purkinje cells may not be specific to the allelic group. Since TH promoter is activated by Ca2+, TH expression in the mutant Purkinje cells may predict neuronal dysfunction caused by alterations in cellular Ca2+currents.
通过膜去极化调节酪氨酸羟化酶基因的表达。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kilbourne,EJ;Nankova,BB;Lewis,EJ;McMahon,A;Osaka,H;Sabban,DB;Sabban,EL
通讯作者：
Sabban,EL