Nucleostemin affects the proliferation but not differentiation of oral squamous cell carcinoma cells
Nucleostemin affects the proliferation but not differentiation of oral squamous cell carcinoma cells
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DOI：
10.1111/j.1349-7006.2011.01935.x
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发表时间：
2011-07
期刊：
影响因子：
5.7
通讯作者：
R. Yoshida;T. Fujimoto;S. Kudoh;M. Nagata;H. Nakayama;M. Shinohara;Takaaki Ito
中科院分区：
文献类型：
--
作者：
R. Yoshida;T. Fujimoto;S. Kudoh;M. Nagata;H. Nakayama;M. Shinohara;Takaaki Ito
据报道，核蛋白 (NS) 对于干细胞和癌细胞的增殖至关重要。为了研究 NS 在口腔鳞状细胞癌 (OSCC) 中的意义，我们检测了舌肿瘤组织和 OSCC 细胞系中的 NS 表达。组织学样本中 Nucleostemin 的表达与 Ki-67 的表达呈正相关。此外，使用 siRNA，NS 表达与 OSCC 细胞系的细胞增殖相关，siRNA 上调 p27（一种细胞周期蛋白依赖性激酶抑制剂）。关于 OSCC 分化，NS 表达不影响角化或口腔上皮分化标志物，如外皮蛋白和细胞角蛋白19。因此，NS在正常和肿瘤性口腔上皮组织中广泛表达，并且可能是增殖的标志物。 （《癌症科学》2011 年；102：1418–1423）
Nucleostemin (NS) has been reported as essential for stem and cancer cell proliferation. To investigate the significance of NS in oral squamous cell carcinomas (OSCCs), we examined NS expression in neoplastic tissue of the tongue and in OSCC cell lines. Nucleostemin expression in the histological samples showed positive correlation with Ki‐67 expression. Furthermore, NS expression was associated with cellular proliferation in OSCC cell lines using siRNA, which upregulated p27, a cyclin‐dependent kinase inhibitor. Regarding OSCC differentiation, NS expression did not influence cornification or oral epithelial differentiation markers such as involucrin and cytokeratin19. Thus, NS is widely expressed in normal and neoplastic oral epithelial tissues, and is likely a marker of proliferation. (Cancer Sci 2011; 102: 1418–1423)