The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior
The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior
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DOI：
10.1101/gad.1010302
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发表时间：
2002-11-15
影响因子：
10.5
通讯作者：
Mattox, W
中科院分区：
文献类型：
--
作者：
Dauwalder, B;Tsujimoto, S;Mattox, W
果蝇体细胞性别决定调控通路已经得到了很好的研究，但很少有人知道它最终控制的靶基因。在一个差异筛选的性别特异性转录表达苍蝇头，我们确定了一个高度男性丰富的转录编码Takeout，一个蛋白质相关的超家族的因素，结合小的亲脂性分子。我们发现，性别特异性的取出转录本来自与成年大脑密切相关的脂肪体组织，并依赖于性别决定基因doublex（dsx）和fruitless（fru）。男性特异性的Doubletex和Fruitless蛋白一起激活Takeout表达，而女性特异性的Doubletex蛋白独立于Fru抑制Takeout。当通常表达takeout的细胞通过表达transformer-F蛋白而雌性化时，雄性求偶行为急剧减少，这表明这些细胞中的雄性身份是行为所必需的。外卖基因中的功能缺失突变减少了雄性求偶，并与无结果突变协同作用，表明外卖与其他涉及雄性交配行为的fru依赖因素起着多余的作用。Takeout序列与果蝇基因组的比较揭示了一个由20个相关分泌因子组成的家族。这些基因的一个子集的表达分析表明，takeout基因家族编码多个因子与性别特异性功能。
The Drosophila somatic sex-determination regulatory pathway has been well studied, but little is known about the target genes that it ultimately controls. In a differential screen for sex-specific transcripts expressed in fly heads, we identified a highly male-enriched transcript encoding Takeout, a protein related to a superfamily of factors that bind small lipophilic molecules. We show that sex-specific takeout transcripts derive from fat body tissue closely associated with the adult brain and are dependent on the sex determination genes doublesex (dsx) and fruitless (fru). The male-specific Doublesex and Fruitless proteins together activate Takeout expression, whereas the female-specific Doublesex protein represses takeout independently of Fru. When cells that normally express takeout are feminized by expression of the Transformer-F protein, male courtship behavior is dramatically reduced, suggesting that male identity in these cells is necessary for behavior. A loss-of-function mutation in the takeout gene reduces male courtship and synergizes with fruitless mutations, suggesting that takeout plays a redundant role with other fru-dependent factors involved in male mating behavior. Comparison of Takeout sequences to the Drosophila genome reveals a family of 20 related secreted factors. Expression analysis of a subset of these genes suggests that the takeout gene family encodes multiple factors with sex-specific functions.