Observations on the changes seen in living cells during growth and division
Observations on the changes seen in living cells during growth and division
复制标题
观察活细胞生长和分裂过程中的变化
DOI：
10.1098/rspb.1922.0050
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发表时间：
1922
期刊：
影响因子：
--
通讯作者：
T. S. Strangeways
中科院分区：
文献类型：
--
作者：
T. S. Strangeways
本文对鸡胚胎和成年鸡组织的体外培养进行了观察。培养物主要为7~9日龄鸡眼脉络膜细胞和成年鸡膝关节软骨细胞。培养的方法是将小块组织包埋在一滴鸡血浆的盖片上，在其中加入一滴鸡胚提取物。将盖片倒置在中空玻璃片上，用熔化的石蜡密封，立即放入39℃的培养箱中，每隔一天传代培养一次。观察到的细胞是在传代培养后在培养箱中生长24小时的培养物中发现的。
The observations recorded in this paper were made on cultures in vitro of embryonic and adult chick tissues. The cultures chiefly studied were choroidal cells from the eyes of seven to nine days’ chick embryos and cartilage cells from knee-joints of adult fowls. The method of cultivation was embedding small fragments of tissue on a coverslip in one drop of chick plasma, to which was added one drop of embryo chick extract. The coverslip was inverted over a hollow glass slide, sealed with melted paraffin wax, and at once placed in an incubator at 39° C. The tissues were sub-cultured every second day. The cells observed were found in cultures which had been growing for 24 hours in the incubator after sub-culture.