Fitness costs of sulfoxaflor resistance in the cotton aphid, Aphis gossypii Glover
Fitness costs of sulfoxaflor resistance in the cotton aphid, Aphis gossypii Glover
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棉蚜 Aphis gossypii Glover 对氟啶虫胺抗性的适应性成本
DOI：
10.1016/j.pestbp.2019.04.009
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发表时间：
2019-07-01
影响因子：
4.7
通讯作者：
Gao, Xiwu
中科院分区：
文献类型：
--
作者：
Ma, Kangsheng;Tang, Qiuling;Gao, Xiwu
棉蚜是一种重要的经济害虫，危害世界各地的多种农作物。中国棉田部分种群对亚磺胺类杀虫剂亚磺胺产生了中等抗药性。为评价磺胺氟草胺抗性对棉铃虫适合度成本的影响，采用年龄阶段两性生命表法比较了亚磺胺抗性品系(SulR)和等基因敏感品系(SS)的生活史特征。结果表明，抗性品系的适合度降低(相对适合度为0.917)，寿命、繁殖力、净繁殖力、平均世代时间和毛繁殖率均显著降低。与敏感品系相比，Suir品系各若虫龄期发育历期较短。此外，Suir品系的成虫产卵前期(APOP)和总产卵前期(TPOP)也显著短于敏感品系。对6个发育和生殖相关基因的研究表明，ECR、USP和JHBP在Suir品系中高表达，而VG的mRNA转录水平显著低于敏感品系。这些结果表明，棉铃虫对磺胺氟草胺的抗性存在适合度成本，ECR、USP、JHBP和VG的不同表达可能在这种权衡中起着非常重要的作用。
Aphis gossypii Glover is an economically important pest of numerous crops throughout the world. Some field populations of A.gossypii in China have developed moderate level of resistance to sulfoxaflor, a newly released sulfoximine insecticide for management of sap-feeding pests. To evaluate the effect of sulfoxaflor resistance on the fitness cost of A. gossypii, the life history traits of sulfoxaflor-resistant strain (SulR) and an isogenic susceptible strain (SS) were compared using the age-stage, two-sex life table approach. The results showed that the resistant strain had a reduction in fitness (relative fitness = 0.917), along with significantly decreases in longevity, fecundity, net reproductive (R-o), mean generation time (T) and gross reproductive rate (GRR). Compared to the susceptible strain, SuIR strain showing a shorter developmental duration of each nymph instar stage. Moreover, the adult pre-oviposition period (APOP) and total preoviposition period (TPOP) of SuIR strain were also significantly shorter than that of the susceptible strain. Investigation of six development and reproduction related genes indicated that EcR, USP and JHBP were overexpressed in the SuIR strain, while the mRNA transcript level of Vg was decreased significantly compared to the susceptible strain. These results suggest that there is a fitness cost associated with sulfoxaflor resistance in A. gossypii and the different expression of EcR, USP, JHBP, and Vg may play very important role in this trade-off.