Direct molecular detection of nucleic acids by fluorescence signal amplification
Direct molecular detection of nucleic acids by fluorescence signal amplification
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DOI：
10.1021/ja053417j
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发表时间：
2005-09-14
影响因子：
15
通讯作者：
Leclerc, M
中科院分区：
文献类型：
--
作者：
Ho, HA;Doré, K;Leclerc, M
本文报道了一种集成的无PCR DNA传感器，它结合了序列特异性受体、光学聚合物传感器和内源荧光放大机制。该传感器是基于静电结合到ss-DNA或ds-DNA时，由阳离子聚噻吩所采用的不同构象，并在所得的荧光聚噻吩/ds-DNA复合物和相邻的荧光团连接到ss-DNA探针之间的有效和快速的能量转移。这种分子系统允许在3毫升的水溶液中，或3 zM，在5分钟内检测只有5个分子。此外，这项工作表明，第一次，直接检测单核苷酸多态性（SNPs）从临床样本在短短几分钟内，而不需要核酸扩增。
An integrated PCR-free DNA sensor, which combines a sequence-specific receptor, an optical polymeric transducer, and an intrinsic fluorescence amplification mechanism, is reported. This sensor is based on the different conformations adopted by a cationic polythiophene when electrostatically bound to ss-DNA or ds-DNA, and on the efficient and fast energy transfer between the resulting fluorescent polythiophene/ds-DNA complex and neighboring fluorophores attached to ss-DNA probes. This molecular system allows the detection of only five molecules in 3 mL of an aqueous solution, or 3 zM, in 5 min. Moreover, this work demonstrates, for the first time, the direct detection of single nucleotide polymorphisms (SNPs) from clinical samples in only a few minutes, without the need for nucleic acid amplification.