Quantitative estimation os bile salts in serum.

Quantitative estimation os bile salts in serum.
复制标题

血清中胆汁盐的定量估计。

DOI:
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发表时间:
1970
期刊:
Canadian Journal of Biochemistry
影响因子:
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通讯作者:
N. Javitt
N. Javitt
中科院分区:
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文献类型:
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作者:
S. Ali;N. Javitt

文献摘要

被引文献

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对现有的气液色谱法测定血清胆盐的方法进行了两大改进。一个新的蛋白质沉淀步骤使用2,2-二甲氧基丙烷几乎完全回收添加的胆汁盐。使用薄层色谱的纯化步骤可以很好地回收单羟基、二羟基和三羟基胆汁酸。胆盐的总回收率大于70%。新方法减少了血清胆盐分析所需的时间。
Two major modifications of existing methods for the quantitative estimation of serum bile salts by gas–liquid chromatography have been made. A new protein precipitation step using 2,2-dimethoxypropane gives almost complete recovery of added bile salts. A purification step using thin-layer chromatography gives good recovery of mono-, di-, and tri-hydroxy bile acids. Overall recovery of bile salts is greater than 70%. The new procedure reduces the time required for serum bile salt analysis.