Nucleotide sequence of the Rickettsia prowazekii citrate synthase gene.
Nucleotide sequence of the Rickettsia prowazekii citrate synthase gene.
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普瓦泽基立克次体柠檬酸合酶基因的核苷酸序列。
DOI：
10.1128/jb.169.8.3564-3572.1987
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发表时间：
1987
影响因子：
3.2
通讯作者：
Krause,DC
中科院分区：
文献类型：
--
作者：
Wood,DO;Williamson,LR;Winkler,HH;Krause,DC
普氏立克次体柠檬酸合成酶(GLTA)基因定位于一条2.0kb的染色体片段。部分片段的DNA序列分析显示，该片段有一个1,308个碱基对的开放阅读框架，编码一个435个氨基酸的蛋白质，相对分子质量为49,171。该翻译产物在大小上与大肠杆菌和猪心柠檬酸合成酶单体以及在含有立克次体基因的大肠杆菌微细胞中合成的蛋白质相当。Prowazekii柠檬酸合成酶的氨基酸序列与大肠杆菌和猪心肌酶的氨基酸序列比较表明，这两种细菌蛋白具有广泛的同源性(59%)。相比之下，只有20%的立克次体酶残留物与功能相似的猪心脏酶残留物共享。在开放阅读框的上游，在彼此靠近的情况下，鉴定了与大肠杆菌RNA聚合酶和核糖体结合的共同序列同源的序列。S1核酸酶定位实验表明，该基因在大肠杆菌中的转录起点位于其上游区域。立克次体GLTA基因中密码子的使用被发现非常偏向，与在大肠杆菌中观察到的模式不同。在立克次体密码子的第三碱基位置优先使用腺嘌呤和尿嘧啶。
The Rickettsia prowazekii citrate synthase (gltA) gene, previously cloned in Escherichia coli, was localized to a 2.0-kilobase chromosomal fragment. DNA sequence analysis of a portion of this fragment revealed an open reading frame of 1,308 base pairs that encodes a protein of 435 amino acids with a molecular weight of 49,171. This translation product is comparable in size to both the E. coli and pig heart citrate synthase monomers and to the protein synthesized in E. coli minicells containing the rickettsial gene. Comparisons between the deduced amino acid sequence of R. prowazekii citrate synthase and those of the E. coli and pig heart enzymes revealed extensive homology (59%) between the two bacterial proteins. In contrast, only 20% of the rickettsial enzyme residues were shared with the functionally similar pig heart enzyme residues. Upstream from the open reading frame and in close proximity to one another, sequences with homology to E. coli consensus sequences for RNA polymerase and ribosome binding were identified. S1 nuclease mapping experiments demonstrated that the start of transcription for this gene in E. coli was located in the upstream region. Codon usage in the rickettsial gltA gene was found to be very biased and differed from the pattern observed in E. coli. Adenine and uracil were used preferentially in the third base position of rickettsial codons.