Biological control of Verticillium wilt on cotton by the use of fluorescent Pseudomonas spp. under field conditions
Biological control of Verticillium wilt on cotton by the use of fluorescent Pseudomonas spp. under field conditions
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DOI：
10.1016/j.biocontrol.2009.11.011
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发表时间：
2010-04-01
影响因子：
4.2
通讯作者：
Benlioglu, Kemal
中科院分区：
文献类型：
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作者：
Erdogan, Oktay;Benlioglu, Kemal
在59种荧光假单胞菌中，根据以下标准选择从棉花和杂草根际收集的菌株：（1）在体外对大丽轮枝菌的抑制，（2）使用大丽轮枝菌分生孢子茎注射对从细菌化种子生长的两个棉花品种的病害抑制，和（3）在温室条件下的幼苗活力测试（干重）。进一步测试了从苍耳（Xanthium strumarium）（FP 22）、马齿苋（Portulaca sp.）（FP 23）、陆地棉（Gossypium hirsitum）（FP 30）和田旋花（Convolvulus arvensis）（FP 35）分离的四种选择的假单胞菌菌株以及已知的生物防治剂Serratia muthica（HRO-C48）对黄萎病、棉花生长参数和天然侵染田间产量的影响。在2005年和2006年进行的试验中，与非细菌化对照相比，使用FP 22、FP 23、FP 30、FP 35和HRO-C48进行种子细菌化的AUDPC降低范围分别为39. 2%至50. 9%和22. 1%至36. 8%。与未处理的对照相比，种子细菌化植物的生长参数（植物高度、主茎上的节数和白色花上方的NAWF节）显著更高。在2005年的田间试验中，用4株假单胞菌和HRO-C48处理沙雅314和阿卡拉玛克夏的籽棉，增产幅度分别为13.1%~ 22.3%和4.2%~ 12.8%。2006年处理对籽棉产量影响不显著。我们的研究结果表明，用我们的假单胞菌处理棉花植物的种子。菌株和已知菌株Serratia dahliae可以帮助生物防治大丽轮枝菌并改善棉田的生长参数。(c)2009 Elsevier Inc. All rights reserved.
Four out of 59 fluorescent Pseudomonas spp. strains collected from cotton and weed rhizosphere were selected based on the following criteria: (1) inhibition of Verticillium dahliae in vitro, (2) disease suppression on two cotton cultivars grown from bacterized seeds using stem-injection with the conidia of V. dahliae, and (3) seedling vigor test (dry weight) under greenhouse conditions. Four selected Pseudomonas strains isolated from Xanthium strumarium (FP22), Portulaca sp. (FP23), Gossypium hirsitum (FP30), and Convolvulus arvensis (FP35), as well as the known biocontrol agent Serratia plymuthica (HRO-C48), were further tested for the impact on Verticillium wilt, growth parameters of cotton, and yield in a naturally infested field. The reduction of AUDPC by the seed bacterization with FP22, FP23, FP30, FP35, and HRO-C48 compared to non-bacterized control ranged from 39.2% to 50.9% and 22.1% to 36.8% in trials done in 2005 and 2006, respectively. The growth parameters (plant height, number of nodes on main stem, and NAWF-nodes above white flower) were significantly higher in seed bacterized plants compared to the untreated control. In the 2005 field trial, the increase of seed cotton yield by the treatment with four Pseudomonas strains and HRO-C48 ranged from 13.1% to 22.3% in Sayar 314 and 4.2% to 12.8% in Acala Maxxa. Seed cotton yield was not significantly influenced by the 2006 treatments. Our results indicate that seed treatment of cotton plants with our Pseudomonas spp. strains and the known strain Serratia plymuthica can help in the biocontrol of V. dahliae and improve growth parameters in cotton fields. (c) 2009 Elsevier Inc. All rights reserved.