JC virus in human glial-derived tumors
JC virus in human glial-derived tumors
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DOI：
10.1016/s0046-8177(00)80256-7
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发表时间：
2000-03-01
期刊：
影响因子：
3.3
通讯作者：
Ferrante, P
中科院分区：
文献类型：
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作者：
Caldarelli-Stefano, R;Boldorini, R;Ferrante, P
为了研究多瘤病毒 JC (JCV)、BK (BKV) 和猿猴多瘤病毒 (SV40) 在人脑肿瘤中的存在和作用，通过分子生物学和免疫组织化学检查了 25 个神经胶质源性肿瘤（10 个星形细胞瘤、5 个室管膜瘤、5 个少突神经胶质瘤和 5 个胶质母细胞瘤）的样本。大T（LT）区巢式PCR及其序列分析显示6例（4例星形细胞瘤、1例少突胶质细胞瘤和1例室管膜瘤）有JCV，而转录控制区（TCR）仅在1例星形细胞瘤、少突胶质细胞瘤和室管膜瘤中扩增，其中1例（星形细胞瘤）免疫组化（JCV LT）、TCR序列分析也呈阳性染色少突胶质细胞瘤的病毒株显示出与已知病毒株无关的 JCV 重排结构，而星形细胞瘤和室管膜瘤则公开了已知可诱发动物脑肿瘤的 JCV Mad-4 病毒株。我们认为 JCV 可能在这些脑肿瘤的发病机制中发挥了作用。版权所有 (C) 2000 W.B.桑德斯公司。
To investigate the presence and the role of polyomaviruses JC (JCV), BK (BKV), and the simian polyomavirus (SV40) in human brain tumors, samples from 25 glial-derived tumors (10 astrocytomas, 5 ependymomas, 5 oligodendrogliomas, and 5 glioblastomas) were examined by means of molecular biology and immunohistochemistry. Nested PCR of the large T (LT) region and its sequence analysis showed JCV in 6 cases (4 astrocytomas, 1 oligodendroglioma, and 1 ependymoma), while the transcriptional control region (TCR) was amplified only in 1 astrocytoma, the oligodendroglioma, and the ependymoma, one of which (astrocytoma) also stained positively by immunohistochemistry (JCV LT), TCR sequence analysis of the oligodendroglioma showed a JCV rearranged structure not related to a known viral strain, while the astrocytoma and the ependymoma disclosed a JCV Mad-4 strain that is known to induce brain tumors in animals. We suggest that JCV could have played a role in the pathogenesis of these brain tumors. Copyright (C) 2000 by W.B. Saunders Company.