Expression of pathogenesis-related genes in cotton stems in response to infection by Verticillium dahliae
Expression of pathogenesis-related genes in cotton stems in response to infection by Verticillium dahliae
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DOI：
10.1006/pmpp.2001.0320
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发表时间：
2001-03
影响因子：
2.7
通讯作者：
H. G. M. C. Fadden;R. Chapple;R. Feyter;E. Dennis
中科院分区：
文献类型：
--
作者：
H. G. M. C. Fadden;R. Chapple;R. Feyter;E. Dennis
摘要研究了土传维管枯萎病菌大丽轮枝菌（Verticilliumdahliae）侵染棉花后，棉花茎中几种病程相关蛋白基因的mRNA转录水平。逆转录PCR用于估计对应于特定转录物的mRNA水平。为了确定基因激活的时间或水平的差异是否可能解释陆地棉品种对黄萎病的易感性差异，我们比较了相对抗性的商业品种Sicala V2与易感品种CS50的基因激活模式。一个非落叶，澳大利亚分离的V. dahliae和感染实现茎穿刺接种与分生孢子悬浮液的病原体。杜松烯合酶、酸性几丁质酶、碱性几丁质酶、β-1，3-葡聚糖酶和至少一个编码潜在PR 10蛋白的棉花Ypr 10基因同源物家族成员的基因的mRNA水平在接种V. dahliae后增加，而苯丙氨酸解氨酶基因的mRNA水平在所有样品中相似。即使叶坏死症状被认为是不太严重的西卡拉V2后，大丽轮枝菌感染，没有差异的时间或在基因表达的激活程度之间观察到的两个品种。在用bion ®（苯并-（1，2，3）-噻二唑-7-硫代甲酸S -甲酯，BTH）或茉莉酮酸甲酯处理CS50后未观察到显著的基因活化，但在bion ®处理后两个品种的叶坏死严重程度降低，这在CS50中是边缘性的，但在Sicala V2中明显。因此，在此观察到的棉花对黄萎病的增强的部分抗性，无论是由于栽培品种还是bion ®处理，都不伴随所研究的基因的表达时间或水平的变化。因此，很可能是其他一些因素造成了所观察到的耐药性程度。
Abstract The levels of mRNA transcripts of genes for several pathogenesis-related proteins were examined in cotton stems after infection of the plants with the soil-borne, vascular wilt pathogen Verticillium dahliae . Reverse transcription PCR was used to estimate mRNA levels corresponding to particular transcripts. In order to determine if differences in timing or levels of gene activation were likely to account for differences in the susceptibility of Gossypium hirsutum cultivars to Verticillium wilt, we compared the gene activation patterns in the relatively resistant commercial cultivar, Sicala V2 with those exhibited by the susceptible cultivar, CS50. A non-defoliating, Australian isolate of V. dahliae was used and infection was achieved by stem-puncture inoculation with a conidial suspension of the pathogen. Levels of mRNA for genes for cadinene synthase, acidic chitinase, basic chitinase, β-1,3-glucanase, and at least one member of the family of cotton Ypr10 gene homologues coding for potential PR10 proteins increased after inoculation with V. dahliae , while levels of mRNA for a gene for phenylalanine ammonia lyase were similar in all samples. Even though leaf necrosis symptoms were found to be less severe in Sicala V2 after V. dahliae infection, no differences in the timing or in the extent of activation of gene expression were observed between the two cultivars used. Significant gene activation was not observed after treatment of CS50 with bion ® (benzo-(1,2,3)-thiadiazole-7-carbothioc acid S -methyl ester, BTH) or methyl jasmonate, but there was a reduction in the severity of leaf necrosis of both cultivars after bion ® treatment, which was marginal in CS50 but clearly evident in Sicala V2. Thus the enhanced partial resistance of cotton to Verticillium wilt observed here, whether due to cultivar or bion ® treatment, is not accompanied by a change in the timing or levels of expression of the genes investigated. Therefore it is likely that some other factors are responsible for the degree of resistance observed.