Heavy ion irradiation induces autophagy in irradiated C2C12 myoblasts and their bystander cells
Heavy ion irradiation induces autophagy in irradiated C2C12 myoblasts and their bystander cells
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DOI：
10.1093/jmicro/dfq059
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发表时间：
2010-12-01
期刊：
影响因子：
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通讯作者：
Yorifuji, Hiroshi
中科院分区：
文献类型：
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作者：
Hino, Mizuki;Hamada, Nobuyuki;Yorifuji, Hiroshi
自噬是细胞内物质降解的主要过程之一。在这里，我们研究了重离子辐照对C2C12小鼠成肌细胞及其非靶向旁观者细胞诱导自噬的潜在影响。在辐照细胞中，超微结构分析显示，在辐照后20分钟内，自噬各阶段（即吞噬细胞、自噬小体和自噬小体）的自噬结构积累。还观察到多泡体（MVBs）和含有MVBs的自溶酶体（两性体）。重离子辐照增加了微管相关蛋白1、轻链3和LysoTracker Red （LTR）的染色。这种增强的染色被自噬抑制剂3-甲基腺嘌呤抑制。除了辐照细胞外，旁观者细胞LTR染色也呈阳性。总之，这些结果表明重离子辐照不仅在受辐照的成肌细胞中诱导自噬，而且在其周围细胞中也诱导自噬。
Autophagy is one of the major processes involved in the degradation of intracellular materials. Here, we examined the potential impact of heavy ion irradiation on the induction of autophagy in irradiated C2C12 mouse myoblasts and their non-targeted bystander cells. In irradiated cells, ultrastructural analysis revealed the accumulation of autophagic structures at various stages of autophagy (i.e. phagophores, autophagosomes and autolysosomes) within 20 min after irradiation. Multivesicular bodies (MVBs) and autolysosomes containing MVBs (amphisomes) were also observed. Heavy ion irradiation increased the staining of microtubule-associated protein 1 light chain 3 and LysoTracker Red (LTR). Such enhanced staining was suppressed by an autophagy inhibitor 3-methyladenine. In addition to irradiated cells, bystander cells were also positive with LTR staining. Altogether, these results suggest that heavy ion irradiation induces autophagy not only in irradiated myoblasts but also in their bystander cells.