EPIDERMODYSPLASIA VERRUCIFORMIS-ASSOCIATED HUMAN PAPILLOMAVIRUS-8 - GENOMIC SEQUENCE AND COMPARATIVE-ANALYSIS
EPIDERMODYSPLASIA VERRUCIFORMIS-ASSOCIATED HUMAN PAPILLOMAVIRUS-8 - GENOMIC SEQUENCE AND COMPARATIVE-ANALYSIS
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DOI：
10.1128/jvi.58.2.626-634.1986
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发表时间：
1986-05-01
影响因子：
5.4
通讯作者：
PFISTER, H
中科院分区：
文献类型：
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作者：
FUCHS, PG;IFTNER, T;PFISTER, H
人乳头瘤病毒 (HPV) 8 会诱发皮肤肿瘤，这种肿瘤具有恶性转化的高风险。 HPV8 的核苷酸序列已被确定，并与具有不同致癌潜力的乳头瘤病毒序列进行比较。 HPV8 基因组的一般组织与其他类型的基因组相似。在开放阅读框 (ORF) E1、E2 和 L1 中发现了高度保守的属特异性序列。在 ORF E6、E7 和 L2 中，HPV8 的亲缘关系较远，但可以区分 HPV8 属于 HPV1-棉尾兔乳头瘤病毒组的亚属。 ORF E4 和部分 ORF L2 内的序列具有相当的类型特异性。 HPV8 具有几个独特的特征：(i) 非编码区的大小显着减小（397 个碱基对），形成复杂二级结构的潜力看似较低； (ii) ORF E1 3''半部分中的一簇推定启动子元件； (iii)靠近ORF E2的C末端的RNA聚合酶III启动子样序列； (iv) 特别令人感兴趣的是 ORF E4 编码的推定蛋白与 Epstein-Barr 病毒核抗原 2 蛋白之间的同源性，这可能反映了病毒介导的转化中的类似机制。
Human papillomavirus (HPV) 8 induces skin tumors which are at high risk for malignant conversion. The nucleotide sequence of HPV8 has been determined and compared to sequences of papillomaviruses with different oncogenic potential. The general organization of the HPV8 genome is similar to that of other types. Highly conserved, genus-specific sequences were found in open reading frames (ORFs) E1, E2 and L1. In ORFs E6, E7 and L2, HPV8 is more distantly related, but it was possible to differentiate subgenera in which HPV8 belonged to the HPV1-cottontail rabbit papillomavirus group. Sequences within ORF E4 and part of ORF L2 are rather type specific. HPV8 stands out by several unique features: (i) the considerably reduced size of the noncoding region (397 base pairs), with a seemingly low potential for forming complex secondary structures; (ii) a cluster of putative promoter elements in the 3'' half of ORF E1; (iii) an RNA polymerase III promoter-like sequence close to the C terminus of ORF E2; and (iv) of particular interest, the homology between the putative protein encoded by ORF E4 and the Epstein-Barr virus nuclear antigen 2 protein, which may reflect similar mechanisms in virus-mediated transformation.