ESTABLISHMENT AND CHARACTERIZATION OF A NEW HUMAN PROSTATIC-CARCINOMA CELL-LINE (DUPRO-1)
ESTABLISHMENT AND CHARACTERIZATION OF A NEW HUMAN PROSTATIC-CARCINOMA CELL-LINE (DUPRO-1)
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DOI：
10.1016/s0022-5347(17)37960-0
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发表时间：
1991-09-01
影响因子：
6.6
通讯作者：
WEBB, KS
中科院分区：
文献类型：
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作者：
GINGRICH, JR;TUCKER, JA;WEBB, KS
用裸鼠移植瘤DU5683建立了一株新的人前列腺癌DUPro-1细胞株。这是通过将分散的异种移植细胞包埋在0.1×5.0厘米内完成的。意大利面条状的基底膜MATRIGEL[BMM(Collaborative Research，Inc.)]，这是一种促进向组织培养过渡的独特技术。经过30多次培养后，细胞呈现贴壁生长和血清浓度无关的生长状态，倍增时间为22~24小时。细胞在BMM涂层培养皿上生长时表现出明显的形态差异，呈假腺体结构，与塑料培养皿上典型的均质单层生长形成鲜明对比。光镜和电子显微镜下可见粘连的间变性上皮细胞，与前列腺癌一致。核型分析显示，所有人类染色体，接近四倍体，10~12个标记，3~4条X染色体，没有Y染色体。细胞注射S.C.在雄性和雌性无瘤小鼠中，DuPro-1细胞具有同样的致瘤性，这表明DuPro-1细胞对激素不敏感。在BMM中植入细胞可能有助于从体外难以启动的肿瘤发展其他组织培养细胞系。DuPro-1为研究前列腺癌的生物学、免疫学和化疗敏感性提供了有价值的手段。
A new human prostate adenocarcinoma cell line (DuPro-1) has been established from the athymic nude mouse supported xenograft DU5683. This was accomplished by embedding dispersed xenograft cells in 0.1 by 5.0 cm. spaghetti-like strands of Basement Membrane MATRIGEL [BMM (Collaborative Research, Inc.)], a unique technique facilitating the transition to tissue culture. Now passed over 30 times, the cells display anchorage and serum concentration independent growth with a doubling time of 22 to 24 hours. Cells exhibit pronounced morphological differences when grown on BMM coated culture dishes, assuming a pseudoglandular configuration, in contrast to typical homogeneous monolayer growth on plastic culture dishes. Light and electron microscopy show cohesive sheets of anaplastic epithelial cells, consistent with prostate carcinoma. Karyotypic analysis revealed all human chromosomes, near tetraploidy, 10 to 12 markers, and 3 to 4 X chromosomes, without a Y chromosome. Cells injected s.c. or embedded in BMM and implanted in the subrenal capsule space are equally tumorigenic in male and female athymic mice, suggesting that DuPro-1 cells are hormonally insensitive. Embedding cells in BMM may be useful in developing other tissue culture cell lines from neoplasms difficult to initiate in vitro. DuPro-1 should provide a valuable means to study the biology, immunology, and chemosensitivity of human prostate cancer.