CO2 and compressive immobilization of C. elegans on-chip
CO2 and compressive immobilization of C. elegans on-chip
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DOI：
10.1039/b807345g
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Chronis, Nikos
中科院分区：
文献类型：
--
作者：
Chokshi, Trushal Vijaykumar;Ben-Yakar, Adela;Chronis, Nikos
我们提出了两种微流控方法固定蛔虫C。芯片上的优雅第一种方法创造了一个二氧化碳微环境，而第二种方法利用可变形的PDMS膜来机械地限制蠕虫的运动。一个片上的“行为”模块被用来描述这些方法对蠕虫运动模式的影响。我们的研究结果表明，这两种方法都适合于短期（分钟）的蠕虫固定。如果需要在固定期间进行荧光成像，则CO2方法提供了长期固定（1-2小时）和减少光漂白的额外优点。我们设想在C.包括细胞发育和神经元再生研究。
We present two microfluidic approaches for immobilizing the roundworm C. elegans on-chip. The first approach creates a CO2 micro-environment while the second one utilizes a deformable PDMS membrane to mechanically restrict the worm's movement. An on-chip 'behavior' module was used to characterize the effect of these methods on the worm's locomotion pattern. Our results indicate that both methods are appropriate for the short-term (minutes) worm immobilization. The CO2 method offers the additional advantages of long-term immobilization (1-2 hours) and reduced photobleaching, if fluorescent imaging during immobilization is required. We envision the use of these methods in a wide variety of biological studies in C. elegans, including cell developmental and neuronal regeneration studies.