ENZYME-LINKED LECTIN ASSAY (ELLA) .2. DETECTION OF CARBOHYDRATE GROUPS ON THE SURFACE OF UNFIXED CELLS
ENZYME-LINKED LECTIN ASSAY (ELLA) .2. DETECTION OF CARBOHYDRATE GROUPS ON THE SURFACE OF UNFIXED CELLS
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DOI：
10.1016/0014-4827(84)90359-8
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发表时间：
1984-01-01
影响因子：
3.7
通讯作者：
GOLDSTEIN, IJ
中科院分区：
文献类型：
--
作者：
MCCOY, JP;VARANI, J;GOLDSTEIN, IJ
建立了一种酶联凝集素测定法（ELLA），用于检测非固定细胞表面的特定碳水化合物单位。由于细胞结合的酶-凝集素偶联物在细胞形成之前被特异性地从细胞中洗脱，因此可以在标准ELISA（酶联免疫吸附试验）板阅读器中读取该分析。ELLA，当在ELISA板读取器中读取时，可以比荧光素偶联凝集素的标准免疫荧光更好地检测和相对定量特定表面碳水化合物单位。
An enzyme-linked lectin assay (ELLA) was developed to detect specific carbohydrate units on the surface of unfixed cells. The assay may be read in standard ELISA [enzyme-linked immunosorbent assay] plate readers, since the cell-bound enzyme-lectin conjugate is specifically eluted from the cells prior to development of the conjugate. ELLA, when read in an ELISA plate reader, allows better detection and relative quantitation of specific surface carbohydrate units than is possible by standard immunofluorescence with fluorescein-conjugated lectins.