MUTATIONS AFFECTING THE PATTERN OF THE LARVAL CUTICLE IN DROSOPHILA-MELANOGASTER .1. ZYGOTIC LOCI ON THE 2ND CHROMOSOME
MUTATIONS AFFECTING THE PATTERN OF THE LARVAL CUTICLE IN DROSOPHILA-MELANOGASTER .1. ZYGOTIC LOCI ON THE 2ND CHROMOSOME
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DOI：
10.1007/bf00848156
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
KLUDING, H
中科院分区：
文献类型：
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作者：
NUSSLEINVOLHARD, C;WIESCHAUS, E;KLUDING, H
为了寻找黑腹果蝇第二染色体上的胚胎致死性突变体，建立了5764个甲烷磺酸乙酯处理的等基因平衡系。在这些品系中，4217个品系携带一个或多个新诱导的致死突变，对应于总共7600个致死突变。取致死性系和未孵化系（2843个系）中25%以上未孵化系的卵，在复合显微镜下进行脱毛、固定、清除和记分，观察幼虫角质层异常。共分离出272个突变体，其表型根据表皮模式与野生型胚胎明确区分。在具有相似表型的突变体之间进行的互补试验中，48个位点被多个等位基因鉴定，平均每个位点有5.4个等位基因。13个突变体与其他所有突变体互补。通过重组分析绘制了互补基团的成员图谱。相似表型位点的聚类不明显。根据等位基因频率的分布和新位点的发现率，估计这61个位点代表了第二条染色体上产生幼虫角质层可识别表型的大多数胚胎致死位点。
In a search for embryonic lethal mutants on the second chromosome ofDrosophila melanogaster, 5764 balanced lines isogenic for an ethyl methane sulfonate (EMS)-treatedcn bw spchromosome were established. Of these lines, 4217 carried one or more newly induced lethal mutations corresponding to a total of 7600 lethal hits. Eggs were collected from lethal-bearing lines and unhatched embryos from the lines in which 25% or more of the embryos did not hatch (2843 lines) were dechorionated, fixed, cleared and scored under the compound microscope for abnormalities of the larval cuticle. A total of 272 mutants were isolated with phenotypes unequivocally distinguishable from wild-type embryos on the basis of the cuticular pattern. In complementation tests performed between mutants with similar phenotype, 48 loci were identified by more than one allele, the average being 5.4 alleles per locus. Complementation of all other mutants was shown by 13 mutants. Members of the complementation groups were mapped by recombination analysis. No clustering of loci with similar phenotypes was apparent. From the distribution of the allele frequencies and the rate of discovery of new loci, it was estimated that the 61 loci represent the majority of embryonic lethal loci on the second chromosome yielding phenotypes recognizable in the larval cuticle.