Systematic Localization of the Arabidopsis Core Cell Cycle Proteins Reveals Novel Cell Division Complexes
Systematic Localization of the Arabidopsis Core Cell Cycle Proteins Reveals Novel Cell Division Complexes
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DOI：
10.1104/pp.109.148643
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发表时间：
2010-02-01
期刊：
影响因子：
7.4
通讯作者：
Russinova, Eugenia
中科院分区：
文献类型：
--
作者：
Boruc, Joanna;Mylle, Evelien;Russinova, Eugenia
细胞分裂取决于细胞周期蛋白依赖激酶的正确定位，这些激酶受磷酸化、细胞周期蛋白水解和蛋白-蛋白相互作用的调节。虽然免疫检测可以确定细胞周期蛋白的丰度和定位，但它们不适合检测细胞分裂过程中分子成分的动态重排。本研究采用活体方法追踪了烟草和拟南芥细胞分裂过程中融合绿色荧光蛋白的60个拟南芥核心细胞周期蛋白的亚细胞定位。在这两个物种中，一些细胞周期蛋白与有丝分裂结构（如浓缩染色体和前期带）表现出动态关联，表明其靶向机制具有很强的保守性。此外，共定位蛋白被证明在体内结合，加强了它们的定位-功能连接。因此，我们确定了未知的时空区域，其中功能细胞周期蛋白相互作用最有可能发生。
Cell division depends on the correct localization of the cyclin-dependent kinases that are regulated by phosphorylation, cyclin proteolysis, and protein-protein interactions. Although immunological assays can define cell cycle protein abundance and localization, they are not suitable for detecting the dynamic rearrangements of molecular components during cell division. Here, we applied an in vivo approach to trace the subcellular localization of 60 Arabidopsis (Arabidopsis thaliana) core cell cycle proteins fused to green fluorescent proteins during cell division in tobacco (Nicotiana tabacum) and Arabidopsis. Several cell cycle proteins showed a dynamic association with mitotic structures, such as condensed chromosomes and the preprophase band in both species, suggesting a strong conservation of targeting mechanisms. Furthermore, colocalized proteins were shown to bind in vivo, strengthening their localization-function connection. Thus, we identified unknown spatiotemporal territories where functional cell cycle protein interactions are most likely to occur.