Identity and public health potential of Cryptosporidium spp. in water buffalo calves in Egypt
Identity and public health potential of Cryptosporidium spp. in water buffalo calves in Egypt
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DOI：
10.1016/j.vetpar.2012.08.015
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发表时间：
2013-01-16
影响因子：
2.6
通讯作者：
Xiao, Lihua
中科院分区：
文献类型：
--
作者：
Amer, Said;Zidan, Shereif;Xiao, Lihua
关于水牛隐孢子虫物种的多样性和公共卫生意义知之甚少。在这项研究中，我们研究了隐孢子虫的分布。在埃及的布法罗小牛身上。采用改良的Ziehl-Neelsen染色法对179头小牛和359名成年牛的直肠粪便标本进行隐孢子虫卵囊显微镜筛查。隐孢子虫属对17份显微镜检查阳性的犊牛标本进行小亚基rRNA基因的DNA序列分析，对隐孢子虫进行60 kDa糖蛋白基因的序列分析。在10份标本中发现ryanae隐孢子虫，C. parvum，前者属于新鉴定的C. ryanae布法罗变异体，后者属于IldA 20 G1亚型（5例）和IlaA 15 G1 R1亚型（2例）。C. ryanae和C. parvum和C. bovis和C. andersoni代表了埃及水牛中隐孢子虫传播的一些特征。由爱思唯尔公司出版
Little is known about the diversity and public health significance of Cryptosporidium species in water buffaloes. In this study, we examined the distribution of Cryptosporidium spp. in water buffalo calves in Egypt. Rectal fecal specimens from 179 calves and 359 adults were screened microscopically for Cryptosporidium oocysts using modified Ziehl-Neelsen stain. Cry ptosporidium spp. in 17 microscopy-positive specimens from calves were genotyped by DNA sequence analysis of the small-subunit rRNA gene, and Cryptosporidium parvum was subtyped by sequence analysis of the 60 kDa glycoprotein gene. Cryptosporidium ryanae was found in 10 specimens and C. parvum in 7 specimens, with the former belonging to the newly identified C. ryanae buffalo variant and the latter belonging to the subtypes IldA20G1 (in 5 specimens) and IlaA15G1R1 (in 2 specimens). The prevailing occurrence of C. ryanae and the subtype family lid of C. parvum and the absence of C. bovis and C. andersoni represent some features of Cryptosporidium transmission in water buffaloes in Egypt. Published by Elsevier B.V.