The Heterologous Expression of the Iris lactea var. chinensis Type 2 Metallothionein IlMT2b Gene Enhances Copper Tolerance in Arabidopsis thaliana
The Heterologous Expression of the Iris lactea var. chinensis Type 2 Metallothionein IlMT2b Gene Enhances Copper Tolerance in Arabidopsis thaliana
复制标题
乳鸢尾变种的异源表达。
DOI：
10.1007/s00128-014-1444-x
复制
发表时间：
2015-02-01
影响因子：
2.7
通讯作者：
Lu, Xiao-Qing
中科院分区：
文献类型：
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作者：
Gu, Chun-Sun;Liu, Liang-Qin;Lu, Xiao-Qing
欧里斯·拉克蒂亚变种。中国乳酸菌(I.Lacta var.)中国)是一种广泛适应的多年生植物，具有很高的耐铜性。探讨金属硫蛋白(MTS)在紫胶耐铜性中的作用。Chinensis)，命名为IlMT2b(GenBank登录号：AB907788)，用RACE-PCR方法克隆。ILMT2b在乳酸菌叶片和根中的表达水平。对铜(Cu)处理的反应是诱导中国草的。IlMT2b在拟南芥中的异位表达增加了转基因植株中铜的浓度，减少了过氧化氢的产生。经50亩和100亩M-Cu处理后，两株转基因幼苗的根长分别是野生型的1.5倍和3倍。这些结果表明，IlMT2b基因可能是铜污染土壤植物修复的一个有用的靶基因。
Iris lactea var. chinensis (I. lactea var. chinensis) is a widely adapted perennial species with a high level of copper tolerance. To evaluate the role of metallothioneins (MTs) in copper tolerance in I. lactea var. chinensis, a full-length cDNA homologue of MT2, designated IlMT2b (GenBank accession No. AB907788), was cloned using the RACE-PCR method. The expression level of IlMT2b in the leaves and roots of I. lactea var. chinensis was induced in response to copper (Cu) treatment. Ectopic expression of IlMT2b in Arabidopsis thaliana increased the Cu concentration and reduced H2O2 production in the transgenic plants. After treatment with 50 and 100 mu M Cu, the root length of two transgenic seedlings was respectively about 1.5- and 3-fold longer than that of the wild-type. Together, these results suggested that IlMT2b may represent a useful target gene for the phytoremediation of Cu-polluted soil.