Oxidative Stress Levels Throughout Pregnancy, at Birth, and in the Neonate
Oxidative Stress Levels Throughout Pregnancy, at Birth, and in the Neonate
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DOI：
10.1177/1099800419858670
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发表时间：
2019-07-08
影响因子：
2.5
通讯作者：
Zimmerman, Matthew C.
中科院分区：
文献类型：
--
作者：
Moore, Tiffany A.;Ahmad, Iman M.;Zimmerman, Matthew C.
背景：氧化应激与不良的围产儿结局有关。关于围产期氧化应激的纵向水平或母体和新生儿氧化应激水平之间的相关性，我们知之甚少。目的：描述和比较围产期的氧化应激，特别是超氧化物歧化酶、过氧化氢酶和谷胱甘肽的水平。研究设计：纵向描述性设计，分别从母胎医学和普通产科诊所抽取医学上高危和低危孕妇(n=140)。采自妊娠12-20周、24-28周和分娩期间的妇女、出生时的脐带和出生后24-72小时的新生儿。使用电子顺磁共振(EPR)光谱测量超氧化物水平；使用商业试剂盒测量抗氧化剂(超氧化物歧化酶、过氧化氢酶和谷胱甘肽)。用非参数方法检验不同时间点氧化应激水平之间的关系。收集妊娠结局。结果：人口学变量、结局变量和母血、脐带血和婴儿的氧化应激水平在医学高危和低危妇女中有所不同。氧化应激测量的描述性模式随时间和风险组之间的不同而不同。时间点之间的显著相关性被注意到，这表明个体内一致性可能存在于整个围产期。然而，这些相关性在每个医疗风险组中并不一致。结论：EPR波谱是一种可行的围产期人群检测方法。结果提供了有关围产期循环超氧化物水平的新信息，并需要进一步研究产前和新生儿氧化应激生理失调之间的潜在关系。
Background: Oxidative stress is associated with poor perinatal outcomes. Little is known regarding the longitudinal levels of oxidative stress in the perinatal period or the correlation between maternal and neonatal oxidative stress levels. Objective: Describe and compare oxidative stress, specifically superoxide, superoxide dismutase, catalase, and glutathione levels, over the perinatal period. Study Design: Longitudinal descriptive design using a convenience sample of medically high- and low-risk pregnant women (n = 140) from a maternal-fetal medicine and general obstetrics practice, respectively. Blood was obtained from women at 12-20 and 24-28 weeks' gestation and during labor, from the umbilical cord at birth, and from neonates at 24-72 hr after birth. Levels of superoxide were measured using electron paramagnetic resonance (EPR) spectroscopy; antioxidants (superoxide dismutase, catalase, and glutathione) were measured using commercial assay kits. Relationships between oxidative stress levels at different time points were examined using nonparametric methods. Pregnancy outcome was collected. Results: Demographic variables, outcome variables, and oxidative stress levels in maternal blood, cord blood, and infants differed between medically high- and low-risk women. Descriptive patterns for oxidative stress measures varied over time and between risk groups. Significant correlations between time points were noted, suggesting intraindividual consistency may exist throughout the perinatal period. However, these correlations were not consistent across each medical risk group. Conclusion: EPR spectroscopy is a feasible method for the perinatal population. Results provide new information on perinatal circulating superoxide levels and warrant further investigation into potential relationships between prenatal and neonatal physiologic dysregulation of oxidative stress.