Substituting ornithine for arginine in total parenteral nutrition eliminates enhanced tumor growth.
Substituting ornithine for arginine in total parenteral nutrition eliminates enhanced tumor growth.
复制标题
在全肠外营养中用鸟氨酸替代精氨酸可以消除肿瘤生长的促进作用。
DOI：
10.1002/jso.2930500307
复制
发表时间：
1992
影响因子：
2.5
通讯作者：
Ota,DM
中科院分区：
文献类型：
--
作者：
GrossieJr,VB;Nishioka,K;Ajani,JA;Ota,DM
全肠外营养(TPN)可能会促进某些肿瘤的生长：这种促进生长与红细胞多胺水平的增加有关。在可移植的Ward结肠癌大鼠模型上，比较了精氨酸和鸟氨酸对肿瘤生长的影响。研究循环中的精氨酸、鸟氨酸、谷氨酰胺和多胺与肿瘤生长的关系。对于自由喂养的大鼠，肿瘤重量的增加与血浆精氨酸水平的线性下降相关，该水平始终低于年龄匹配的非肿瘤(NTB)大鼠；鸟氨酸和赖氨酸水平不受影响。随后的实验表明，荷瘤大鼠的血浆谷氨酰胺水平也较低。配对喂养的NTB大鼠血浆中精氨酸水平降低，但谷氨酰胺水平没有下降。用精氨酸或以鸟氨酸代替精氨酸两个水平(等摩尔[Orm-Em]或等氮[Orn-IN])的TPN方案对结肠癌荷瘤大鼠进行为期8天的治疗。含精氨酸方案组大鼠最终肿瘤重量(19.8±5.7g，n=4)显著高于自由喂养组(12.1±3.3g，n=6)(P<；0.05)。Orn-EM组(11.2±2.6g，n=4)和Orn-IN组(11.6±0.8g，n=6)的最终瘤重与饲料对照组相似。与对照组相比，当方案中存在精氨酸时，血浆精氨酸水平升高。Orn-EM组和Orn-IN组大鼠血浆精氨酸水平均低于对照组。精氨酸对血浆鸟氨酸水平无影响，但随着TPN中鸟氨酸水平的升高而升高。当方案中存在精氨酸时，血浆谷氨酰胺水平降低，但当鸟氨酸取代精氨酸时，血浆谷氨酰胺水平升高。当精氨酸或鸟氨酸被包括在TPN方案中时，红细胞腐胺增加。这些结果表明，虽然肠外方案中的精氨酸刺激肿瘤生长，但在TPN中用鸟氨酸代替精氨酸并不能促进可移植的结肠癌的生长。©1992 Wiley-Liss，Inc.
Total parenteral nutrition (TPN) may enhance the growth of some tumors: this enhanced growth is associated with an increase in the erythrocyte polyamine levels. The effect of arginine in TPN on tumor growth was compared with ornithine using rats with a transplantable Ward colon tumor. The relationship of circulating arginine, ornithine, glutamine, and polyamines with tumor growth was investigated. For rats fed chow ad libitum, increasing tumor weights were associated with a linear decrease in the plasma arginine levels which was consistently lower than that of age‐matched non‐tumor bearing (NTB) rats; ornithine and lysine levels were not affected. Subsequent experiments suggest that plasma glutamine levels were also lower in tumor bearing rats. Pair‐fed NTB rats had reduced arginine but not glutamine levels in plasma. TPN regimens with arginine or with ornithine substituted for arginine at two levels (equimolar [Orm‐Em] or isonitrogenous [Orn‐IN]) were given to colon tumor bearing rats for 8 days. The final tumor weight of rats which received the arginine‐containing regimen (19.8 ± 5.7 g, n = 4) (P< 0.05) was significantly greater than the tumor weight of rats fed chow ad libitum (12.1 ± 3.3 g, n = 6). The final tumor weights of Orn‐EM (11.2 ± 2.6 g, n = 4) or Orn‐IN (11.6 ± 0.8 g, n = 6) were similar to the chow‐fed controls. The plasma arginine levels were elevated, compared with the control, when arginine was present in the regimen. The plasma arginine levels of rats which received Orn‐EM or Orn‐IN were lower than the controls. The plasma ornithine levels were not affected by arginine in the regimen but were elevated with increasing levels of ornithine in TPN. Plasma glutamine levels were decreased when arginine was present in the regimen but were elevated when ornithine was substituted for arginine. Erythrocyte putrescine was increased when either arginine or ornithine was included in the TPN regimens. These results demonstrate that while arginine in a parenteral regimen stimulates tumor growth, substituting ornithine for arginine in TPN does not enhance the growth of a transplantable colon tumor. © 1992 Wiley‐Liss, Inc.