Dual modality instrument for simultaneous optical coherence tomography imaging and fluorescence spectroscopy
Dual modality instrument for simultaneous optical coherence tomography imaging and fluorescence spectroscopy
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DOI：
10.1117/1.1695564
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发表时间：
2004-05-01
影响因子：
3.5
通讯作者：
Tumlinson, A
中科院分区：
文献类型：
--
作者：
Barton, JK;Guzman, F;Tumlinson, A
我们开发了一种双模装置，结合了光学相干断层扫描（OCT）的解剖成像能力和激光诱导荧光（LIF）光谱的功能能力。OCT提供组织结构的横截面图像，深度可达2毫米，分辨率约为10毫米。LIF光谱学以从给定组织位置发射光谱的形式提供组织化学信息。OCT子系统采用中心波长为1300 nm的超发光二极管，而氦镉激光器提供波长为325和442 nm的LIF激发源。初步数据是在8个死后主动脉样本上获得的，每个样本的长度为10毫米。OCT图像和LIF光谱提供了主动脉壁正常和动脉粥样硬化部分的互补信息。OCT图像显示内膜、中膜、内部弹性层和纤维化区等结构。发射光谱比为520/490 （325 nm激发）和595/635 （442 nm激发），可用于识别正常和斑块区域，正确率分别为97%和91%。随着输送探针的小型化和系统速度的提高，这种双模装置可以为动脉粥样硬化斑块的识别和表征提供有价值的工具。(C) 2004光学仪器工程师学会。
We develop a dual-modality device that combines the anatomical imaging capabilities of optical coherence tomography (OCT) with the functional capabilities of laser-induced fluorescence (LIF) spectroscopy. OCT provides cross-sectional images of tissue structure to a depth of up to 2 mm with approximately 10-mum resolution. LIF spectroscopy provides histochemical information in the form of emission spectra from a given tissue location. The OCT subsystem utilizes a superluminescent diode with a center wavelength of 1300 nm, whereas a helium cadmium laser provides the LIF excitation source at wavelengths of 325 and 442 nm. Preliminary data are obtained on eight postmortem aorta samples, each 10 mm in length. OCT images and LIF spectra give complementary information from normal and atherosclerotic portions of aorta wall. OCT images show structures such as intima, media, internal elastic lamina, and fibrotic regions. Emission spectra ratios of 520/490 (325-nm excitation) and 595/635 (442-nm excitation) could be used to identify normal and plaque regions with 97 and 91% correct classification rates, respectively. With miniaturization of the delivery probe and improvements in system speed, this dual-modality device could provide a valuable tool for identification and characterization of atherosclerotic plaques. (C) 2004 Society of Photo-Optical Instrumentation Engineers.