Photodynamic Therapy Resistant Human Colon Carcinoma HT29 Cells Show Cross‐Resistance to UVA but not UVC Light
Photodynamic Therapy Resistant Human Colon Carcinoma HT29 Cells Show Cross‐Resistance to UVA but not UVC Light
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光动力疗法耐药的人结肠癌 HT29 细胞对 UVA 表现出交叉耐药性，但对 UVC 没有交叉耐药性
DOI：
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发表时间：
2007
影响因子：
3.3
通讯作者：
A. Rainbow
中科院分区：
文献类型：
--
作者：
Natalie J. Zacal;A. Rainbow
The isolation of photodynamic therapy (PDT)‐resistant HT29 human colon adenocarcinoma cells has been reported previously. These PDT‐resistant variants show increased expression of the Hsp27 and BNip3 proteins and a decreased expression of mutant p53 protein compared with parental HT29 cells. Because mutant p53 and increased expression of Hsp27 have been associated with resistance to various chemotherapeutic agents, whereas BNip3 is a potent inducer of apoptosis, we were interested in determining whether these PDT‐resistant cells were cross‐resistant to other cytotoxic agents. In the present report, we examined the colony survival of the PDT‐resistant HT29 variants and several other clonal variants of HT29 cells to ultraviolet light (UV) treatment. The HT29 PDT‐resistant variants showed cross‐resistance to long‐wavelength UVA (320–400 nm) but not to short‐wavelength UVC (200–280 nm) light. Cell sensitivity to UVA or UVC was then correlated with Hsp27, BNip3 and mutant p53 protein levels in the PDT‐resistant variants as well as in several clonal variants of HT29 cells that express different levels of Hsp27, BNip3 and mutant p53. We show that increased expression of Hsp27 and BNip3 and decreased expression of mutant p53 correlated with increased resistance to UVA. In contrast, increased expression of Hsp27 and BNip3 correlated with increased sensitivity to UVC, whereas increased expression of mutant p53 showed no significant correlation with sensitivity to UVC. These results suggest that the PDT‐resistant HT29 cell variants are differentially sensitized to UVA compared with UVC due, in part at least, through the altered expression levels of BNip3, Hsp27 and mutant p53.
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DOI：
10.1046/j.1523-1747.1998.00319.x
发表时间：
1998-09
期刊：
The Journal of investigative dermatology
影响因子：
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作者：
Y. Wang;B. Rosenstein;S. Goldwyn;X Zhang-;M. Lebwohl;H. Wei
通讯作者：
Y. Wang;B. Rosenstein;S. Goldwyn;X Zhang-;M. Lebwohl;H. Wei
平均灭活剂量是衡量细胞放射敏感性的良好指标吗？
影响因子：
3.4
作者：
Tucker,SL
通讯作者：
Tucker,SL
DOI：
--
发表时间：
1990-12
期刊：
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
影响因子：
--
作者：
P. Hinds;C. Finlay;R. S. Quartin;Suzanne J. Baker;E. Fearon;Bert Vogelstein;A. Levine
通讯作者：
P. Hinds;C. Finlay;R. S. Quartin;Suzanne J. Baker;E. Fearon;Bert Vogelstein;A. Levine
HSP27 磷酸化介导的抗氧化应激诱导的肌动蛋白断裂和细胞死亡的抵抗力。
影响因子：
11.2
作者：
Huot,J;Houle,F;Spitz,DR;Landry,J
通讯作者：
Landry,J
影响因子：
8
作者：
Dorothy J. Park;H. Nakamura;A. Chumakov;J. Said;C. Miller;Dan-Lin Chen;H. Koeffler
通讯作者：
Dorothy J. Park;H. Nakamura;A. Chumakov;J. Said;C. Miller;Dan-Lin Chen;H. Koeffler