Platelet-derived growth factor-modulated translatable mRNAs.
Platelet-derived growth factor-modulated translatable mRNAs.
复制标题
血小板衍生生长因子调节的可翻译 mRNA。
DOI：
10.1128/mcb.3.8.1478-1487.1983
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发表时间：
1983
影响因子：
5.3
通讯作者：
Scher,CD
中科院分区：
文献类型：
--
作者：
Hendrickson,SL;Scher,CD
用电泳均一或高纯度的血小板衍生生长因子(PDGF)处理密度受阻的BALB/c 3T3细胞，可刺激经无细胞翻译鉴定的几种丰富的mRNA的快速和选择性积累。这些可翻译的mRNA在进入S阶段之前很久就出现了。与PDGF调节的DNA合成相比，选择性地积累mRNA所需的PDGF更少。在加入表皮生长因子后，可翻译的mRNAs也会积累，但在添加胰岛素或缺乏血小板的血浆后则不会积累。它们的选择性积累被放线菌素D阻断。定义了三类PDGF调节的mRNAs。PDGF加入后30~60min内出现早期(初级)RNA；放线菌亚胺不能阻断其积累。另一个早期的mRNA也在60min内出现，但PDGF和放线菌亚胺都需要处理才能获得最佳的积累。第三类是次级RNA，在90到120分钟后开始积累；这一类的出现被放线菌亚胺抑制。翻译产物的一维和双向凝胶电泳表明，自发转化的BALB/c 3T3(ST2-3T3)细胞系不需要PDGF或表皮生长因子就能结构性地积累次级生长因子调节的mRNAs。这些可翻译的mRNAs的积累可能是PDGF调节DNA合成所必需的。
The treatment of density-arrested BALB/c 3T3 cells with electrophoretically homogeneous or highly purified preparations of the platelet-derived growth factor (PDGF) stimulated the rapid and selective accumulation of several species of abundant mRNA identified by cell-free translation. These translatable mRNAs appeared long before entry into the S phase. Less PDGF was required for selective mRNA accumulation than for PDGF-modulated DNA synthesis. The translatable mRNAs also accumulated after addition of the epidermal growth factor but not after addition of insulin or platelet-poor plasma. Their selective accumulation was blocked by addition of actinomycin D. Three classes of PDGF-modulated mRNAs were defined. An early (primary) RNA appeared within 30 to 60 min of PDGF addition; its accumulation was not blocked by cycloheximide. Another early mRNA also appeared within 60 min, but treatment with both PDGF and cycloheximide was required for optimal accumulation. A third class, secondary RNAs, began to accumulate later at 90 to 120 min; the appearance of this class was inhibited by cycloheximide. One- and two-dimensional gel electrophoresis of translation products demonstrated that a spontaneously transformed BALB/c 3T3 (ST2-3T3) cell line, which does not require PDGF or epidermal growth factor for growth, constitutively accumulated the secondary growth factor-regulated mRNAs. The accumulation of these translatable mRNAs may be required for PDGF-modulated DNA synthesis.
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影响因子：
12.4
作者：
Kariko, Katalin;Muramatsu, Hiromi;Welsh, Frank A.;Ludwig, Janos;Kato, Hiroki;Akira, Shizuo;Weissman, Drew
通讯作者：
Weissman, Drew
DOI：
10.1016/j.omtm.2020.05.030
发表时间：
2020-09-11
影响因子：
4.7
作者：
Anttila, Vesa;Saraste, Antti;Gan, Li-Ming
通讯作者：
Gan, Li-Ming
DOI：
10.1056/nejmoa2035389
发表时间：
2021-02-04
期刊：
The New England journal of medicine
影响因子：
--
作者：
Baden LR;El Sahly HM;Essink B;Kotloff K;Frey S;Novak R;Diemert D;Spector SA;Rouphael N;Creech CB;McGettigan J;Khetan S;Segall N;Solis J;Brosz A;Fierro C;Schwartz H;Neuzil K;Corey L;Gilbert P;Janes H;Follmann D;Marovich M;Mascola J;Polakowski L;Ledgerwood J;Graham BS;Bennett H;Pajon R;Knightly C;Leav B;Deng W;Zhou H;Han S;Ivarsson M;Miller J;Zaks T;COVE Study Group
通讯作者：
COVE Study Group
DOI：
10.1016/j.jconrel.2015.08.007
发表时间：
2015-11-10
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Pardi N;Tuyishime S;Muramatsu H;Kariko K;Mui BL;Tam YK;Madden TD;Hope MJ;Weissman D
通讯作者：
Weissman D
影响因子：
5.4
作者：
Semple SC;Leone R;Barbosa CJ;Tam YK;Lin PJC
通讯作者：
Lin PJC