Odorant responses of dual polarity are mediated by cAMP in mouse olfactory sensory neurons
Odorant responses of dual polarity are mediated by cAMP in mouse olfactory sensory neurons
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DOI：
10.1152/jn.00140.2004
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发表时间：
2004-09-01
影响因子：
2.5
通讯作者：
Restrepo, D
中科院分区：
文献类型：
--
作者：
Delay, R;Restrepo, D
一些嗅觉感觉神经元（OSNs）对气味的反应具有超极化。虽然兴奋性反应的转导是通过环核苷酸门控（CNG）通道的开放介导的，但抑制性反应的机制仍存在争议。我们发现，小鼠OSNs响应气味的去极化或超极化反应在松散的补丁测量。在穿孔的补丁配置，OSNs不仅响应与CNG通道介导的兴奋一致的电流，但也表现出增强的外向电流，与抑制反应一致。增加cAMP水平可引起不同OSN的兴奋性或抑制性反应。此外，来自CNG通道的CNGA 2亚基缺陷的小鼠的OSN显示既不兴奋也不抑制反应。因此，CNG通道介导抑制性嗅觉反应。
Some olfactory sensory neurons (OSNs) respond to odors with hyperpolarization. Although transduction for excitatory responses is mediated by opening of a cyclic nucleotide-gated (CNG) channel, there is controversy on the mechanism underlying inhibitory responses. We find that mouse OSNs respond to odorants by either depolarizing or hyperpolarizing responses in loose-patch measurements. In the perforated-patch configuration, OSNs not only responded with a current consistent with CNG channel-mediated excitation but also displayed enhancement of outward currents, consistent with inhibitory responses. Increasing cAMP levels pharmacologically elicited excitatory or inhibitory responses in different OSNs. In addition, OSNs from mice defective for the CNGA2 subunit of the CNG channel displayed neither excitatory nor inhibitory responses. Thus CNG channels mediate inhibitory olfactory responses.