Molecularly imprinted polymer-coated hollow fiber membrane for the microextraction of triazines directly from environmental waters
Molecularly imprinted polymer-coated hollow fiber membrane for the microextraction of triazines directly from environmental waters
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DOI：
10.1016/j.chroma.2016.03.004
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发表时间：
2016-04-15
影响因子：
4.1
通讯作者：
Martin-Esteban, Antonio
中科院分区：
文献类型：
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作者：
Barahona, Francisco;Diaz-Alvarez, Myriam;Martin-Esteban, Antonio
在这项工作中，新的分子印迹聚合物涂层的中空纤维（MIP-HFs）已被制备和评估的发展的微固相萃取方法，用于分析三嗪在水溶液中的样品，使用高效液相色谱和紫外检测。该方法将液液微萃取技术与分子印迹技术相结合。简而言之，甲苯薄膜固定在所得MIP-HF的孔中。然后，将经调节的MIP-HF浸入水样中。在搅拌一定时间下，目标分析物从样品中液-液萃取到固定化甲苯中，然后这些扩散到MIP的特异性结合位点。研究了时间、搅拌速度、盐析效果等因素对再胶凝效果的影响，确定了最佳的再胶凝条件。在100 mL纯水中，加标15 g L ~（-1）三嗪类化合物，回收率在0.8- 6.9%之间，相对标准偏差（RSD）< 10%（n=3），检出限（LOD）在0.05-0.1 pg L ~（-1）之间。所提出的方法被成功地应用于提取三嗪从加标自来水和河水样品在μ g L-1浓度水平。使用开发的MIP-HFs的微萃取程序克服了水介质中MIPs典型的低性能和缺乏选择性识别的问题，使得可以在预期的真实的浓度水平下测定环境沃茨水中的三嗪。(C)2016爱思唯尔B. V.保留所有权利。
In this work, novel molecularly imprinted polymer-coated hollow fibers (MIP-HFs) have been prepared and evaluated for the development of a micro-solid phase extraction method for the analysis of triazines in aqueous samples using high performance liquid chromatography and UV detection. The proposed extraction method combines liquid-liquid microextraction and molecular imprinting technology. In brief, a thin film of toluene is immobilised in the pores of the obtained MIP-HF. Afterwards, the conditioned MIP-HF is immersed in the water sample. Under stirring for a certain time, the target analytes are liquid-liquid extracted from the sample to the immobilised toluene and then these diffuse to the specific binding sites of the MIP. The effect of various experimental parameters as time and stirring-rate and salting-out effect among others, were studied for the establishment of optimum rebinding conditions. Recoveries for seven triazines tested in 100 mL pure water samples spiked with 15 g L-1 of each triazine were within 0.8-6.9%, with a relative standard deviation (RSD) < 10% (n=3), The detection limits (LODs) were within 0.05-0.1 pg L-1, depending upon the triazine. The proposed methodology was successfully applied to extract the triazines from spiked tap and river water samples at mu g L-1 concentration level. The mircro extraction procedure with the developed MIP-HFs overcomes the typical low performance and lack of selective recognition of MIPs in aqueous media, allowing the determination of triazines in environmental waters at expected real concentration levels. (C) 2016 Elsevier B.V. All rights reserved.