A SIMPLE, NONCHROMATOGRAPHIC PURIFICATION PROCEDURE FOR MONOCLONAL-ANTIBODIES - ISOLATION OF MONOCLONAL-ANTIBODIES AGAINST CYTOCHROME P450 ISOZYMES
A SIMPLE, NONCHROMATOGRAPHIC PURIFICATION PROCEDURE FOR MONOCLONAL-ANTIBODIES - ISOLATION OF MONOCLONAL-ANTIBODIES AGAINST CYTOCHROME P450 ISOZYMES
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DOI：
10.1016/0022-1759(87)90180-3
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发表时间：
1987-06-26
影响因子：
2.2
通讯作者：
THOMAS, PE
中科院分区：
文献类型：
--
作者：
REIK, LM;MAINES, SL;THOMAS, PE
本文介绍了一种从小鼠腹水中纯化单克隆抗体的简便方法。该方法快速、廉价且具有高容量，包括用辛酸沉淀污染蛋白质，然后用硫酸铵沉淀免疫球蛋白。该两步法显示出对于纯化各种免疫球蛋白（包括IgG1、IgG2a和IgG2b）有效。在本报告中，30多个单克隆抗体针对细胞色素P450同工酶已被纯化，并通过这种方法进行了表征。
A simple, non-chromatographic purification procedure for monoclonal antibodies from mouse ascites fluid is described. This procedure, which is rapid, inexpensive, and has high capacity involves the precipitation of contaminating proteins with caprylic acid followed by precipitation of immunoglobulin using ammonium sulfate. This two-step procedure is shown to be effective for the purification of various immunoglobulins including IgG1, IgG2a and IgG2b. In the present report, more than 30 monoclonal antibodies directed against cytochrome P450 isozymes have been purified by this method and characterized.