A plant phytosulfokine peptide initiates auxin-dependent immunity through cytosolic Ca2+ signaling in tomato.
A plant phytosulfokine peptide initiates auxin-dependent immunity through cytosolic Ca2+ signaling in tomato.
复制标题
植物植物硫因子肽通过番茄中的胞质 Ca2 信号传导启动生长素依赖性免疫。
作者：
Zhang H;Hu ZJ;Lei C;Zheng CF;Wang J;Shao SJ;Li X;Xia XJ;Cai XZ;Zhou J;Zhou YH;Yu JQ;Foyer CH;Shi K
植物磺因子（PSK）是一种二硫酸化五肽，是一种重要的信号分子。尽管它最近被认为与植物防御病原体感染有关，但所涉及的机制仍然知之甚少。使用表面等离振子共振和基因沉默方法，我们发现番茄（Solanum lycopersicum）PSK 受体 PSKR1（而不是 PSKR2）在免疫反应中充当主要的 PSK 受体。 PSK 信号基因的沉默使番茄更容易受到经济上重要的坏死性病原体灰葡萄孢的感染。对防御激素生物合成或信号传导缺陷的番茄突变体的分析表明，PSK 诱导的免疫需要生长素生物合成和相关的防御途径。在这里，使用表达水母发光蛋白的番茄植物，我们提供了证据，证明番茄 PSKR1 感知 PSK 升高了胞质 [Ca2+]，通过增强钙调蛋白和生长素生物合成 YUC 之间的结合活性，导致生长素依赖性免疫反应。因此，我们的数据表明，PSK 作为一种损伤相关分子模式发挥作用，主要由 PSKR1 感知，PSKR1 增加胞质 [Ca2+] 并激活生长素介导的途径，从而增强番茄植物对 B 的免疫力。灰霉病。
Phytosulfokine (PSK) is a disulfated pentapeptide that is an important signaling molecule. Although it has recently been implicated in plant defenses to pathogen infection, the mechanisms involved remain poorly understood. Using surface plasmon resonance and gene silencing approaches, we showed that the tomato (Solanum lycopersicum) PSK receptor PSKR1, rather than PSKR2, functioned as the major PSK receptor in immune responses. Silencing of PSK signaling genes rendered tomato more susceptible to infection by the economically important necrotrophic pathogenBotrytis cinerea. Analysis of tomato mutants defective in either defense hormone biosynthesis or signaling demonstrated that PSK-induced immunity required auxin biosynthesis and associated defense pathways. Here, using aequorin-expressing tomato plants, we provide evidence that PSK perception by tomato PSKR1 elevated cytosolic [Ca2+], leading to auxin-dependent immune responses via enhanced binding activity between calmodulins and the auxin biosynthetic YUCs. Thus, our data demonstrate that PSK acts as a damage-associated molecular pattern and is perceived mainly by PSKR1, which increases cytosolic [Ca2+] and activates auxin-mediated pathways that enhance immunity of tomato plants toB. cinerea.