Endogenous prostaglandins regulate spontaneous contractile activity of uterine strips isolated from non-pregnant pigs
Endogenous prostaglandins regulate spontaneous contractile activity of uterine strips isolated from non-pregnant pigs
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DOI：
10.1016/j.prostaglandins.2006.08.003
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发表时间：
2006-12-01
影响因子：
2.9
通讯作者：
Taneike, Tetsuro
中科院分区：
文献类型：
--
作者：
Cao, Jinshan;Kitazawa, Takio;Taneike, Tetsuro
从非妊娠猪分离的子宫肌条显示自发收缩活动。在本研究中，参与内源性洋地黄素在调节子宫自发收缩的机械，免疫组织化学和生物化学方法进行了研究。免疫组化和Western blot分析表明，考克斯-1主要表达于非妊娠猪子宫肌层，呈肌层依赖性（纵肌>环肌），而考克斯-2不表达。用吲哚美辛和选择性考克斯-1抑制剂（SC-560和FR 122047）预处理子宫条显著降低了纵向肌肉自发收缩的幅度和频率，但考克斯抑制剂的抑制作用在环形肌肉中可以忽略不计。另一方面，考克斯-2抑制剂CAY 10404不改变任一肌肉层的自发收缩。SC-560预处理降低子宫肌层PGF（2 α）和PGE（2）水平。收缩性FP和EP 3受体以肌层依赖性方式（纵向肌>环形肌）表达，类似于考克斯-1的表达模式。总之，由考克斯-1产生的内源性前列腺素选择性地调节非妊娠猪子宫纵肌的自发收缩活动，这是由于收缩性前列腺素受体和COX-L的异质性表达。(c)2006爱思唯尔公司All rights reserved.
Myometrial strips isolated from non-pregnant pigs show spontaneous contractile activity. In the present study, the involvement of endogenous prostaglandins in regulation of uterine spontaneous contraction was investigated using mechanical, immunohistochemical and biochemical approaches. Immunohistochemical study and Western blot analysis for immunoreactive cyclooxygenase (COX) indicated that COX-1 but not COX-2 was expressed predominantly in the myometrium of non-pregnant pigs in a muscle layer-dependent manner (longitudinal muscle > circular muscle). Pretreatment of uterine strips with indomethacin and selective COX-1 inhibitors (SC-560 and FR122047) significantly reduced both the amplitude and frequency of spontaneous contraction in the longitudinal muscle, but inhibition by COX inhibitors was negligible in the circular muscle. On the other hand, CAY10404, a COX-2 inhibitor, did not change the spontaneous contraction in either of the muscle layers. Pretreatment with SC-560 reduced myometrial PGF(2 alpha) and PGE(2) levels. Contractile FP and EP3 receptors were expressed in a muscle layer-dependent manner (longitudinal muscle > circular muscle), similar to the expression pattern of COX-1. In conclusion, endogenous prostaglandins produced by COX-1 regulate spontaneous contractile activity of non-pregnant porcine uterine longitudinal muscle selectively due to the heterogeneous expression of contractile prostanoid receptors and COX-L. (c) 2006 Elsevier Inc. All rights reserved.