Resistance-related physiological response of rice leaves to the compound stress of enhanced UV-B radiation and Magnaporthe oryzae
Resistance-related physiological response of rice leaves to the compound stress of enhanced UV-B radiation and Magnaporthe oryzae
复制标题
水稻叶片对增强UV-B辐射和稻瘟病菌复合胁迫的抗性相关生理反应
DOI：
10.1080/17429145.2018.1478007
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发表时间：
2018-05-23
影响因子：
3.2
通讯作者：
Yue, Ming
中科院分区：
文献类型：
--
作者：
He, Yongmei;Li, Xiang;Yue, Ming
在稻瘟病菌侵染前、侵染期间和侵染后分别给予增强的UV-B辐射(5.0kJm(-2))。研究了UV-B辐射和稻曲霉单独及复合胁迫对水稻叶片抗性生理和基因表达的影响。结果表明，在米曲霉感染前给予UV-B辐射(UV-BM.)显著提高了病程相关蛋白苯丙氨酸解氨酶(PAL)、脂氧合酶(LOX)、几丁质酶(CHT)和-1，3-葡聚糖酶(CHT)的活性，提高了抗病相关物质(类黄酮和总酚)含量和抗病相关基因(OsPAL和OsCHT)的表达，从而提高了水稻叶片的抗病性。同时暴露于UV-B辐射和米曲霉(UV-B/M)。显著增加OsLOX2的表达和PR活性。米曲霉感染后暴露于UV-B辐射(M.UV-B)降低了类黄酮类化合物的含量，但没有提高PR活性，并增加了OsLOX2的表达。UV-BM、UV-B/M和M.UV-B复合处理使病情指数分别降低62.3%、40.2%和26.6%，说明UV-B辐射对稻瘟病菌的发生有抑制作用，但在稻瘟病菌侵染后提供UV-B辐射后，抑制作用减弱。因此，水稻对UV-B辐射和稻曲霉复合胁迫的响应是通过与胁迫发生顺序相关的抗性相关的生理机制实现的。
An enhanced UV-B radiation (5.0kJm(-2)) was supplied before, during, and after Magnaporthe oryzae infection. The effects of single and compound stress of the UV-B radiation and M.oryzae on the resistance physiology and gene expression of rice leaves were examined. Results revealed that UV-B radiation given before M.oryzae infection (UV-BM.) significantly increased the pathogenesis-related proteins (PRs) activities of phenylalanine ammonialyase (PAL), lipoxygenase (LOX), chitinase (CHT), and -1,3-glucanase, the resistance-related substances (flavonoids and total phenols) content, and resistance-related genes (OsPAL and OsCHT) expression, thereby improving the disease resistance of rice leaves. Simultaneous exposure to UV-B radiation and M.oryzae (UV-B/M.) significantly increased the OsLOX2 expression and the PRs activities. Exposure to UV-B radiation after M.oryzae infection (M.UV-B) decreased the flavonoid content, did not improve the PRs activity, and increased OsLOX2 expression. Compound treatments of UV-BM., UV-B/M., and M.UV-B reduced the disease index by 62.3%, 40.2%, and 26.6%, respectively, indicating UV-B radiation inhibited the occurrence of M.oryzae disease, but its inhibitory effect weakened when it was provided after M.oryzae infection. Hence, rice responded to the compound stress of UV-B radiation and M.oryzae through a resistance-related physiological mechanism associated with the sequence of stress occurrence.