Effects of systemic administration of lidocaine and QX-314 on hyperexcitability of spinal dorsal horn neurons after incision in the rat
Effects of systemic administration of lidocaine and QX-314 on hyperexcitability of spinal dorsal horn neurons after incision in the rat
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DOI：
10.1016/j.pain.2006.01.004
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发表时间：
2006-05-01
期刊：
影响因子：
7.4
通讯作者：
Namiki, Akiyoshi
中科院分区：
文献类型：
--
作者：
Kawamata, Mikito;Sugino, Shigekazu;Namiki, Akiyoshi
尽管全身性利多卡因已被证明可以在临床环境中抑制术后疼痛，但利多卡因的作用机制尚未阐明。因此，本研究旨在确定中枢和外周部位对利多卡因对大鼠切口诱导的脊髓背角（SDH）神经元过度兴奋作用的相对贡献。在后躯 RF 中心制作穿过皮肤、筋膜和肌肉的 1 cm 纵向切口之前和之后，记录 SDH 单个宽动态范围 (WDR) 神经元的感受野 (RF) 面积、自发活动和对非伤害性和伤害性刺激的反应。切口后 30 分钟观察到自发活动、RF 大小以及 WDR 神经元对非伤害性和伤害性刺激的反应显着增加 (P < 0.001)。全身给予利多卡因（1 mg/kg 推注加 0.5 mg/kg/h 和 2 mg/kg 推注加 1 mg/kg/h）和 QX-314（1 mg/kg 推注加 0.5 mg/kg/h 和 2 mg/kg 推注加 1 mg/kg/h）显着但暂时抑制和逆转自发活动、对无害和有害刺激和 RF 的反应的增加尺寸（P < 0.01）。在未做切口的假手术动物中，全身施用相同剂量的利多卡因和 QX-314 不会影响 WDR 神经元对无害或有害刺激的反应或其 RF 大小。结果表明，利多卡因的全身给药主要通过外周作用部位对术后疼痛具有抑制作用。 (c) 2006 年国际疼痛研究协会。由 Elsevier B.V. 出版。保留所有权利。
Although systemic lidocaine has been shown to suppress postoperative pain in a clinical setting, the mechanisms of action of lidocaine have not been elucidated. The present study was therefore designed to determine the relative contribution of central and peripheral sites to the action of lidocaine on incision-induced hyperexcitation of spinal dorsal horn (SDH) neurons in the rat. Receptive field (RF) areas, spontaneous activities, and responses of single wide-dynamic-range (WDR) neurons of the SDH to nonnoxious and noxious stimuli were recorded before and after longitudinal incisions of 1 cm through the skin, fascia, and muscle had been made in the center of their RFs of the hindquarters. Significant increases in spontaneous activities, RF sizes, and responses of WDR neurons to both nonnoxious and noxious stimuli were observed at 30 min after the incision (P < 0.001). Systemic administration of lidocaine (1 mg/kg bolus plus 0.5 mg/kg/h and 2 mg/kg bolus plus 1 mg/kg/h) and QX-314 (1 mg/kg bolus plus 0.5 mg/kg/h and 2 mg/kg bolus plus 1 mg/kg/h) significantly but temporarily suppressed and reversed the increases in spontaneous activity, responses to nonnoxious, and noxious stimuli and RF sizes (P < 0.01). Systemic administration of the same doses of lidocaine and QX-314 did not affect responses of WDR neurons to nonnoxious or noxious stimuli or their RF sizes in sham-operated animals in which an incision had not been made. The results suggest that systemic administration of lidocaine has suppressive effects on postoperative pain mainly through peripheral sites of action. (c) 2006 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.