Kinetics of functionalised carbon nanotube distribution in mouse brain after systemic injection: Spatial to ultra-structural analyses.
Kinetics of functionalised carbon nanotube distribution in mouse brain after systemic injection: Spatial to ultra-structural analyses.
复制标题
DOI：
10.1016/j.jconrel.2015.12.039
复制
发表时间：
2016-02-28
期刊：
影响因子：
--
通讯作者：
Al-Jamal KT
中科院分区：
文献类型：
--
作者：
Wang JT;Rubio N;Kafa H;Venturelli E;Fabbro C;Ménard-Moyon C;Da Ros T;Sosabowski JK;Lawson AD;Robinson MK;Prato M;Bianco A;Festy F;Preston JE;Kostarelos K;Al-Jamal KT
早期的研究证明了使用化学功能化的多壁碳纳米管（f-MWNTs）作为大脑纳米载体的成功。很少深入了解f-MWNTs在体内的脑分布动力学的报道。本研究采用了广泛的定性和定量技术，目的是阐明静脉注射后f-MWNT的脑分布。γ-闪烁照相术定量了研究的放射性标记的f-MWNT在全脑实质和毛细血管中的摄取，而3D-单光子发射计算机断层扫描/计算机断层扫描成像和放射自显影术显示了不同脑区域内的空间分布。拉曼和多光子发光与透射电子显微镜一起证实了小鼠脑中存在完整的f-MWNT，在一个无标记的方式。结果表明，除了脑内皮细胞外，小鼠脑实质中也存在f-MWNT。在进一步应用于神经学治疗之前，需要关于区域和细胞脑分布的速率和程度的此类信息。
Earlier studies proved the success of using chemically functionalised multi-walled carbon nanotubes (f-MWNTs) as nanocarriers to the brain. Little insight into the kinetics of brain distribution of f-MWNTs in vivo has been reported. This study employed a wide range of qualitative and quantitative techniques with the aim of shedding the light on f-MWNT's brain distribution following intravenous injection. γ-Scintigraphy quantified the uptake of studied radiolabelled f-MWNT in the whole brain parenchyma and capillaries while 3D-single photon emission computed tomography/computed tomography imaging and autoradiography illustrated spatial distribution within various brain regions. Raman and multiphoton luminescence together with transmission electron microscopy confirmed the presence of intact f-MWNT in mouse brain, in a label-free manner. The results evidenced the presence of f-MWNT in mice brain parenchyma, in addition to brain endothelium. Such information on the rate and extent of regional and cellular brain distribution is needed before further implementation into neurological therapeutics can be made.