Initiation of human immunodeficiency virus type 1 (HIV-1) transcription is inhibited by noncytolytic CD8 suppression.
Initiation of human immunodeficiency virus type 1 (HIV-1) transcription is inhibited by noncytolytic CD8 suppression.
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DOI：
10.2174/1874357900701010001
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发表时间：
2007-01-01
期刊：
影响因子：
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通讯作者：
Tomaras, Georgia D
中科院分区：
文献类型：
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作者：
Overman, R Glenn;Llorens, Anthony L;Tomaras, Georgia D
人类免疫缺陷病毒1型（HIV-1）的复制可以通过非细胞溶解性CD8（+）T细胞介导的抑制来抑制，这是一种特异性靶向HIV-1基因表达的免疫反应。临床研究表明，这种免疫反应可能在宿主防御HIV感染中发挥重要作用。在这项研究中，我们研究了非细胞溶解性CD8（+）T细胞抑制病毒基因表达的不同步骤。利用富含CD4（+）的外周血单核细胞的HIV-1初次感染系统作为相关的离体系统来检查HIV-1生命周期。从两个HIV（+）长期非进展者建立的CD8（+）T细胞系用于检查HIV基因组转录起始和延伸水平的差异。该感染系统与新转录的RNA转录物的实时测量结果相结合，确定短细胞内病毒RNA转录物显著减少（5 - 8倍）。这些数据强烈支持在非细胞溶解性CD8（+）T细胞介导的抑制中启动病毒转录的作用。
The replication of human immunodeficiency virus type 1 (HIV-1) can be inhibited by noncytolytic CD8(+) T cell mediated suppression, an immune response that specifically targets HIV-1 gene expression. Clinical studies demonstrate that this immune response may play an important role in the host defense against HIV infection. In this study, we examined the distinct steps in viral gene expression for inhibition by noncytolytic CD8(+) T cells. A primary HIV-1 infection system of CD4(+) enriched peripheral blood mononuclear cells was utilized to examine the HIV-1 life cycle as a relevant ex vivo system. Established CD8(+) T cell lines from two HIV(+) long-term nonprogressors were used to examine differences at the level of transcriptional initiation and elongation of the HIV genome. This infection system coupled with the results from real-time measurement of newly transcribed RNA transcripts determined that there was a significant decrease (5-8 fold) in short intracellular viral RNA transcripts. These data strongly favor a role for the initiation of virus transcription in noncytolytic CD8(+) T cell mediated suppression.