Identification of distinct K+ channels in mouse spermatogenic cells and sperm
Identification of distinct K+ channels in mouse spermatogenic cells and sperm
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DOI：
10.1017/s0967199402002241
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发表时间：
2002-05-01
期刊：
影响因子：
1.7
通讯作者：
Darszon, A
中科院分区：
文献类型：
--
作者：
Felix, R;Serrano, CJ;Darszon, A
钾离子通道被认为调节哺乳动物精子受精能力的获得。然而，这些蛋白质在精子中的分子身份尚未阐明。在这份报告中，使用免疫共聚焦和电子显微镜，我们发现，至少有四个不同类别的K+通道（Kv1.1，Kv1.2，Kv3.1和GIRK1）的存在和区域分布在小鼠附睾精子的表面。此外，使用逆转录聚合酶链反应的RNA从小鼠生精细胞允许扩增的多个转录本对应的通道，通过免疫细胞化学。与此相一致，全细胞膜片钳记录显示，至少有两种不同的外向整流K+电流在生精细胞的表达。
Potassium (K+) channels are believed to regulate mammalian sperm acquisition of fertilising capacity. However, the molecular identity of these proteins in sperm has not been elucidated. In this report, using immunoconfocal and electron microscopy we show that a minimum of four different classes of K+ channels (Kv1.1, Kv1.2, Kv3.1 and GIRK1) are present and regionally distributed over the surface of mouse epididymal sperm. In addition, the use of reverse transcription polymerase chain reaction on RNA from mouse spermatogenic cells allowed the amplification of multiple transcripts corresponding to the channels identified by immunocytochemistry. Consistent with this, whole-cell patch-clamp recordings showed the expression of at least two different outwardly rectifying K+ currents in spermatogenic cells.