Role of nerve growth factor in the development of rat sympathetic neurons in vitro. I. Survival, growth, and differentiation of catecholamine production.
Role of nerve growth factor in the development of rat sympathetic neurons in vitro. I. Survival, growth, and differentiation of catecholamine production.
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DOI：
10.1083/jcb.75.3.694
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发表时间：
1977-12
期刊：
影响因子：
--
通讯作者：
Patterson PH
中科院分区：
文献类型：
--
作者：
Chun LL;Patterson PH
为了研究神经生长因子（NGF）对神经元存活、生长和分化的影响，将几乎不含其他细胞类型的分离的新生大鼠交感神经元培养物维持3-4周。在没有NGF的情况下，神经元存活时间不超过一天。神经生长因子水平的增加增加神经元的存活和生长（总蛋白和总脂质磷酸盐）;饱和发生在0.5微克/毫升7S神经生长因子。通过测量酪氨酸产生的儿茶酚胺（CA）检测的神经元分化也依赖于培养基中的NGF水平。随着NGF浓度的升高，每个神经元、每纳克蛋白质或每皮摩尔脂质磷酸盐的CA产量增加，直到在1和5微克/毫升7S NGF之间达到饱和。因此，NGF以剂量依赖性方式诱导神经元存活、生长和CA产生的分化。神经元的生长和分化进行了定量比较的存在下，高分子量和低分子量形式的神经生长因子，没有发现显着的功能差异。
To study the effect of nerve growth factor (NGF) on neuronal survival, growth, and differentiation, cultures of dissociated neonatal rat sympathetic neurons virtually free of other cell types were maintained for 3-4 wk. In the absence of NGF, the neurons did not survive for more than a day. Increased levels of NGF increased neuronal survival and growth (total protein and total lipid phosphate); saturation occurred at 0.5 microgram/ml 7S NGF. Neuronal differentiation examined by measuring catecholamine (CA) production from tyrosine also depended on the level of NGF in the culture medium. As the NGF concentration was raised, CA production per neuron, per nanogram protein, or per picomole lipid phosphate increased until saturation was achieved between 1 and 5 microgram/ml 7S NGF. Thus, NGF induces neuronal survival, growth, and differentiation of CA production in a dose-dependent fashion. Neuronal growth and differentiation were quantitatively compared in the presence of the high and low molecular weight forms of NGF; no significant functional differences were found.