Vascular endothelium leaves fingerprints on the surface of erythrocytes
Vascular endothelium leaves fingerprints on the surface of erythrocytes
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DOI：
10.1007/s00424-013-1288-y
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发表时间：
2013-10-01
影响因子：
4.5
通讯作者：
Oberleithner, Hans
中科院分区：
文献类型：
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作者：
Oberleithner, Hans
红细胞（RBC）通过血管的滑动是由位于RBC和内皮细胞（EC）表面的带负电荷的糖萼介导的。在各种血管病变中，EC逐渐失去这一保护性表面层。因此，红细胞与血管内皮密切接触。假设RBC糖萼可能受到EC糖萼不良的不利影响。通过用原子力显微镜技术评价RBC和EC表面层来检验这一假设。在第一系列的实验中，EC单层培养中生长暴露于有节奏的阻力施加的血液覆盖（阻力处理），此后，EC表面的厚度和厚度进行了研究。在第二个系列中，EC单层的糖萼被带负电荷的硫酸乙酰肝素的酶促裂解干扰，然后进行拖曳力处理，并在此之后，RBC表面进行了评价。在第三个系列中，血液覆盖物的RBC糖萼在拖曳力处理之前被酶促干扰，此后，评价EC表面。RBC与EC表面性质之间存在强正相关（r（2）= 0.95）。酶促影响的EC糖萼导致RBC糖萼脱落，反之亦然。结论：血液与内皮细胞之间存在物理相互作用。显然，RBC糖萼反映了EC糖萼的性质。这一观察结果可能对心血管疾病的诊断和治疗产生重大影响。
Gliding of red blood cells (RBC) through blood vessels is mediated by the negatively charged glycocalyx located on the surfaces of both RBC and endothelial cells (EC). In various vasculopathies, EC gradually lose this protective surface layer. As a consequence, RBC come into close physical contact with the vascular endothelium. It is hypothesized that the RBC glycocalyx could be adversely affected by a poor EC glycocalyx. This hypothesis was tested by evaluating the RBC and EC surface layers with atomic force microscopy techniques. In the first series of experiments, EC monolayers grown in culture were exposed to rhythmic drag forces exerted from a blood overlay (drag force treatment), and thereafter, the EC surface was investigated in terms of thickness and adhesiveness. In the second series, the glycocalyx of the EC monolayers was disturbed by enzymatic cleavage of negatively charged heparan sulfates before drag force treatment, and thereafter, the RBC surface was evaluated. In the third series, the RBC glycocalyx of the blood overlay was enzymatically disturbed before drag force treatment, and thereafter, the EC surface was evaluated. A strong positive correlation between the RBC and EC surface properties was found (r (2) = 0.95). An enzymatically affected EC glycocalyx lead to the shedding of the RBC glycocalyx and vice versa. It is concluded that there is physical interaction between the blood and endothelium. Apparently, the RBC glycocalyx reflects properties of the EC glycocalyx. This observation could have a significant impact on diagnosis and treatment of cardiovascular diseases.