Advancements in G protein-coupled receptor biosensors to study GPCR-G protein coupling.
Advancements in G protein-coupled receptor biosensors to study GPCR-G protein coupling.
复制标题
研究 GPCR-G 蛋白偶联的 G 蛋白偶联受体生物传感器的进展。
DOI：
10.1111/bph.15962
复制
发表时间：
2023
影响因子：
7.3
通讯作者：
English,JustinG
中科院分区：
文献类型：
--
作者：
Olsen,ReidHJ;English,JustinG
酶和细胞信号生物传感器被用来破译复杂生物系统的活动。G蛋白偶联受体(GPCRs)是人体内用药最多的一类蛋白质，用于监测G蛋白偶联受体(GPCRs)的生物传感器种类繁多，用途、形式和功能各异。它们的应用不断扩大了我们对这一重要蛋白质类别的理解。在这里，我们简要地总结了这一领域中具有加速重要性的一个子集：测量受体偶联和选择性的传感器生物传感器，重点是测量受体结合和异三聚体信号复合体激活的传感器。
Enzymatic and cellular signalling biosensors are used to decipher the activities of complex biological systems. Biosensors for monitoring G protein‐coupled receptors (GPCRs), the most drugged class of proteins in the human body, are plentiful and vary in utility, form and function. Their applications have continually expanded our understanding of this important protein class. Here, we briefly summarize a subset of this field with accelerating importance: transducer biosensors measuring receptor‐coupling and selectivity, with an emphasis on sensors measuring receptor association and activation of heterotrimeric signalling complexes.