Disposable biosensor based on cathodic electrochemiluminescence of tris(2,2-bipyridine)ruthenium(II) for uric acid determination
Disposable biosensor based on cathodic electrochemiluminescence of tris(2,2-bipyridine)ruthenium(II) for uric acid determination
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DOI：
10.1016/j.aca.2013.01.045
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发表时间：
2013-04-03
影响因子：
6.2
通讯作者：
Capitan-Vallvey, L. F.
中科院分区：
文献类型：
--
作者：
Ballesta-Claver, J.;Rodriguez-Gomez, R.;Capitan-Vallvey, L. F.
描述了一种基于三(2,2-联吡啶)钌(II)-尿酸酶体系阴极ECL猝灭的尿酸(UA)测定新方法。该生物传感器基于双层设计，其中第一层为电化学固定在石墨丝网印刷细胞上的三(2,2-联吡啶)钌(II) (Ru(bpy)(3)(2+))，第二层为壳聚糖中的尿酸酶。尿酸生物传感基于尿酸在尿酸酶存在下固定化Ru(bpy)(3)(2+)引起的阴极ECL上产生的ECL猝灭。使用 1 秒的 -1.1 V 脉冲和 10 秒的停留时间可以估计初始酶速率，并将其用作分析信号。 Stern-Volmer 型校准功能显示的动态范围为 1.0 x 10(-5) 至 1.0 x 10(-3) M，检测限为 3.1 x 10(-6) M，相对标准偏差精度为 13.6% (1.0 x 10(-4) M，n=5)。尿液样本获得了令人满意的结果，为尿酸测定创造了一种经济实惠的替代方案。 (C) 2013 Elsevier B.V. 保留所有权利。
A new method for uric acid (UA) determination based on the quenching of the cathodic ECL of the tris(2,2-bipyridine)ruthenium(II)-uricase system is described. The biosensor is based on a double-layer design containing first tris(2,2-bipyridine)ruthenium(II) (Ru(bpy)(3)(2+)) electrochemically immobilized on graphite screen-printed cells and uricase in chitosan as a second layer. The uric acid biosensing is based on the ECL quenching produced by uric acid over the cathodic ECL caused by immobilized Ru(bpy)(3)(2+) in the presence of uricase. The use of a -1.1 V pulse for 1 s with a dwelling time of 10 s makes it possible to estimate the initial enzymatic rate, which is used as the analytical signal. The Stern-Volmer type calibration function shows a dynamic range from 1.0 x 10(-5) to 1.0 x 10(-3) M with a limit of detection of 3.1 x 10(-6) M and an accuracy of 13.6% (1.0 x 10(-4) M, n=5) as relative standard deviation. Satisfactory results were obtained for urine samples, creating an affordable alternative for uric acid determination. (C) 2013 Elsevier B.V. All rights reserved.