Parkin-co-regulated gene (PACRG) product interacts with tubulin and microtubules
Parkin-co-regulated gene (PACRG) product interacts with tubulin and microtubules
复制标题
DOI：
10.1016/j.febslet.2008.02.081
复制
发表时间：
2008-04-30
期刊：
影响因子：
3.5
通讯作者：
Ikeda, Takashi
中科院分区：
文献类型：
--
作者：
Ikeda, Takashi
Parkin共同调节基因(PACRG)是一种与帕金森病相关的Parkin/Park2基因具有双向启动子的基因。最近，PACRG基因产物被认为与PACRG的功能有关。阿盖拉。然而，它的确切功能仍不清楚。在这里，我评估了PACRG和微管蛋白之间的相互作用。共沉淀实验表明，PACRG直接与微管和α/β-微管蛋白杂二聚体高亲和力结合。显微镜研究表明，PACRG与未聚合的微管蛋白二聚体捆绑在一起，形成分枝状聚集体。PACRG微管结合区的氨基酸序列在各种生物中高度保守，提示微管蛋白结合是PACRG的一种基本性质。结构概要：Mint-6439731，Mint-6439753，Mint-6439768：PACRG(GenBank_Protein_Gi：169219363)，微管蛋白α(uniprotkb：P02550)，微管蛋白β(uniprotkb：Q2XVP4)结合(MI：0407)Mint-6439796：PACRG(GenBank_Protein_Gi：169193263)，微管蛋白α(uniprotkb：P02550)，微管蛋白β(uniprotkb：Q2XVP4)通过下拉(MI：0096)结合(MI：0407)(C)2008欧洲生化学会联合会。爱思唯尔出版公司版权所有。
Parkin-co-regulated gene (PACRG) is a gene that shares a bidirectional promoter with Parkinson's disease-related Parkin/Park2 gene. Recently, the PACRG gene product was implicated in the function of. agella. However, its exact function remains unknown. Here, I assessed the interaction between PACRG and tubulin. Co-sedimentation experiments revealed that PACRG directly binds to microtubules and alpha/beta-tubulin heterodimers with high affinity. Microscopic studies showed that PACRG bundles microtubules and forms branched aggregates with unpolymerized tubulin dimers. The amino acid sequence of the microtubule-binding region of PACRG is highly conserved among various organisms, suggesting that tubulin binding is a basic property of PACRG.Structured summary:MINT-6439731, MINT-6439753, MINT-6439768:PACRG ( genbank_protein_gi: 169219363), tubulin alpha (uniprotkb: P02550), tubulin beta ( uniprotkb: Q2XVP4) bind (MI: 0407) by cosedimentation ( MI: 0027)MINT-6439796:PACRG ( genbank_protein_gi: 169219363), tubulin alpha ( uniprotkb: P02550), tubulin beta ( uniprotkb: Q2XVP4) bind ( MI: 0407) by pull-down ( MI: 0096) (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.