Kinetics of continuous cultivation of the oleaginous yeast Rhodosporidium toruloides.
Kinetics of continuous cultivation of the oleaginous yeast Rhodosporidium toruloides.
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DOI：
10.1016/j.jbiotec.2013.08.010
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发表时间：
2013-10
影响因子：
4.1
通讯作者：
Hongwei Shen;Zhiwei Gong;Xiaobing Yang;Guojie Jin;F. Bai;Z. Zhao
中科院分区：
文献类型：
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作者：
Hongwei Shen;Zhiwei Gong;Xiaobing Yang;Guojie Jin;F. Bai;Z. Zhao
微生物脂是生物燃料和油脂化工行业潜在的替代原料。产油酵母toruloidas2.1389是一种优良的产脂菌。为了获得了解脂质生产过程的参数，我们在碳和氮限制下进行了连续培养实验。在碳限制条件下，该酵母的维持系数为5.7 mg葡萄糖/g细胞/h，最大细胞质量产量为0.42 g细胞/g葡萄糖。在氮限制条件下，稀释率为0.02 h−1时，脂质产率最高，为0.19 g/g；稀释率为0.08 h−1时，比脂形成率最高，为0.058 g/g细胞/h。建立了稳态条件下脂质形成的动力学模型，估计了参数，并预测了最佳连续培养条件。这些数据应该对开发和设计更有效的微生物脂质生产生物工艺非常有帮助。
Microbial lipids are potential alternative feedstock for biofuel and oleochemical industries. The oleaginous yeastRhodosporidium toruloidesAS 2.1389 is an excellent lipid producer. To attain parameters for the understanding of the lipid production process, we performed continuous cultivation experiments under either carbon or nitrogen limitation. The maintenance coefficient and maximum cell mass yield for this yeast were determined as 5.7 mg glucose/g cell/h and 0.42 g cell/g glucose, respectively, under carbon limitation. Under nitrogen limitation, the highest lipid yield of 0.19 g/g was observed at the dilution rate of 0.02 h−1while the highest specific lipid formation rate of 0.058 g/g cell/h at the dilution rate of 0.08 h−1. A kinetic model of lipid formation under steady state conditions was developed, parameters estimated, and optimal continuous cultivation conditions forecasted. These data should be very helpful to develop and design more efficient bioprocesses for microbial lipid production.