Potential of electrospun chitosan fibers as a surface layer in functionally graded GTR membrane for periodontal regeneration
Potential of electrospun chitosan fibers as a surface layer in functionally graded GTR membrane for periodontal regeneration
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DOI：
10.1016/j.dental.2016.10.003
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发表时间：
2017-01-01
期刊：
影响因子：
5
通讯作者：
Rehman, Ihtesham Ur
中科院分区：
文献类型：
--
作者：
Qasim, Saad B.;Najeeb, Shariq;Rehman, Ihtesham Ur
目标。由于破坏性牙周病而丢失的牙周组织的再生仍然是临床医生面临的挑战。引导组织再生（GTR）已成为最广泛应用的再生程序。本研究的目的是制备低纤维取向或高纤维取向的壳聚糖（CH）膜，并考察其作为GTR膜的表面层的适用性，通过控制细胞生长方向来促进与牙周组织的结合。制备了ch掺杂聚乙烯氧化物（PEO）（比例为95:5）的静电纺丝溶液。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、膨胀比、拉伸测试和pH分析、重量谱、紫外可见（UV-vis）光谱和FTIR分析监测降解，对其生物理化和力学性能进行了表征。用人骨肉瘤（MG63）和人胚胎干细胞来源的间充质祖细胞（hES-MP）评估获得的纤维的活力和基质沉积。得到随机和排列的CH纤维。FTIR分析显示静电纺丝前后的CH光谱轮廓整齐。电太阳垫有利于细胞附着，活力随时间增加而增加。纤维通过hES-MPs支持基质沉积。组织学切片显示细胞浸润。面层起到封闭作用，防止连接上皮落入缺损部位，从而维持骨再生的空间。(C) 2016年牙科材料学会。Elsevier Ltd.出版。版权所有。
Objective. The regeneration of periodontal tissues lost as a consequence of destructive periodontal disease remains a challenge for clinicians. Guided tissue regeneration (GTR) has emerged as the most widely practiced regenerative procedure. Aim of this study was to electrospin chitosan (CH) membranes with a low or high degree of fiber orientation and examines their suitability for use as a surface layer in GTR membranes, which can ease integration with the periodontal tissue by controlling the direction of cell growth.Methods. A solution of CH-doped with polyethylene oxide (PEO) (ratio 95:5) was prepared for electrospinning. Characterization was performed for biophysiochemical and mechanical properties by means of scanning electron microscopy (SEM), Fourier Transform Infrared (FTIR) spectroscopy, swelling ratio, tensile testing and monitoring degradation using pH analysis, weight profile, ultraviolet-visible (UV-vis) spectroscopy and FTIR analysis. Obtained fibers were also assessed for viability and matrix deposition using human osteosarcoma (MG63) and human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells.Results. Random and aligned CH fibers were obtained. FTIR analysis showed neat CH spectral profile before and after electrospinning. Electropsun mats were conducive to cellular attachment and viability increased with time. The fibers supported matrix deposition by hES-MPs. Histological sections showed cellular infiltration as well.Significance. The surface layer would act as seal to prevent junctional epithelium from falling into the defect site and hence maintain space for bone regeneration. (C) 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.