Proteomics analysis of normal and senescent NG108-15 cells: GRP78 plays a negative role in cisplatin-induced senescence in the NG108-15 cell line.
Proteomics analysis of normal and senescent NG108-15 cells: GRP78 plays a negative role in cisplatin-induced senescence in the NG108-15 cell line.
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DOI：
10.1371/journal.pone.0090114
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Li W;Wang W;Li Y;Wang W;Wang T;Li L;Han Z;Wang S;Ma D;Wang H
Accelerated senescence (ACS) leading to proliferative arrest is a physiological mechanism of the DNA damage response that occurs during tumor therapy. Our experiment was designed to detect unknown genes that may play important roles in cisplatin-induced senescence and to illustrate the related senescence mechanism. Using 2-dimension electrophoresis (2-DE), we identified 5 protein spots with different expression levels in the normal and senescent NG108-15 cells. According to MALDI-TOF MS analysis, the 5 proteins were determined to be peptidylprolyl isomerase A (PPIA), peroxiredoxin 1 (PRX1), glutathione S-transferase mu 1 (GSTM1), vimentin (VIM) and glucose-regulated protein 78 (GRP78). Then, we investigated how cisplatin-induced senescence was mediated by GRP78 in the NG108-15 cells. Knockdown of GRP78 significantly increased P53 expression in NG108-15 cells. Additionally, 2-deoxy-D-glucose (2DG)-induced GRP78 overexpression protected the NG108-15 cells from cisplatin-induced senescence, which was accompanied by the obvious suppression of P53 and p-CDC2 expression. Inhibition of Ca2+ release from endoplasmic reticulum (ER) stores was also found to be associated with the anti-senescence effect of 2DG-induced GRP78 overexpression. In conclusion, we found 5 proteins that were differentially expressed in normal NG108-15 cells and senescent NG108-15 cells. GRP78 plays an important role in cisplatin-induced senescence in NG108-15 cells, mainly through its regulation of P53 expression and ER calcium efflux.
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影响因子：
2.7
作者：
Gannon HS;Donehower LA;Lyle S;Jones SN
通讯作者：
Jones SN
影响因子：
8
作者：
Al-Bahlani, S.;Fraser, M.;Wong, A. Y. C.;Sayan, B. S.;Bergeron, R.;Melino, G.;Tsang, B. K.
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DOI：
10.1126/science.1189157
发表时间：
2010-11-26
期刊：
Science (New York, N.Y.)
影响因子：
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DOI：
10.1124/jpet.112.197590
发表时间：
2012-12-01
影响因子：
3.5
作者：
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通讯作者：
Gewirtz, David A.
影响因子：
4.8
作者：
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通讯作者：
Wadhwa, R