Diosgenin, a Plant-Derived Sapogenin, Exhibits Antiviral Activity in Vitro against Hepatitis C Virus
Diosgenin, a Plant-Derived Sapogenin, Exhibits Antiviral Activity in Vitro against Hepatitis C Virus
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DOI：
10.1021/np100578u
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发表时间：
2011-04-01
影响因子：
5.1
通讯作者：
Hsu, John T. -A.
中科院分区：
文献类型：
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作者：
Wang, Ya-Jean;Pan, Kao-Lu;Hsu, John T. -A.
Diosgenirr（3 β-羟基-5-螺烯，1）;一种植物来源的皂苷元，用作膳食补充剂。然而，1与病毒复制相关的生物学效应尚未研究。在这项研究中，1对丙型肝炎病毒（HCV）复制的影响进行了评估。基于一个基于酶的HCV亚基因组复制子系统，发现1在低微摩尔浓度下抑制HCV复制。1的EC 50（抑制50% HCV复制的浓度）为3.8 μ M，在该浓度下未观察到细胞毒性。薯蓣皂苷元（1）还显著降低了病毒、RNA和病毒蛋白的水平，分别通过定量实时逆转录酶PCR和Western印迹分析进行评估。此外，在与HCV感染和生命周期中所涉及的所有步骤更密切相关的HCV抗病毒系统中，1在20 μ M时完全消除了HCV复制。此外，1减少信号转导和转录激活因子3的磷酸化。1：和干扰素-α的组合对所得的抗HCV活性产生累加效应。
Diosgenirr (3 beta-hydroxy-5-spirostene, 1); a plant-derived sapogenin, is used as a dietary: supplement However, the biological effects of 1 related to viral replication remain unexplored. In this study, the effects of 1 on hepatitis C virus (HCV) replication were evaluated. Based on a reporter-based HCV subgenomic replicon system, 1 was found to inhibit HCV replication at low micromolar concentrations. The EC50 (concentration at which 50% of HCV replication is inhibited) of 1 was 3.8 mu M No cellular toxicity was observed at this concentration.,Diosgenin, (1) also significantly reduced the levels of viral,RNA and viral proteins as evaluated by quantitative real-time reverse :transcriptase PCR and Western blot analysis, respectively. In addition, in an HCV antiviral system more closely aligned to all steps involved in the HCV infection and life cycle, 1 totally abolished HCV replication at 20 mu M. Moreover, 1 reduced the phosphorylation of signal transducer and activator of transcription 3. A combination of 1:and interferon-alpha exerted an additive effect on the resultant anti-HCV activity.