Molecular epidemiology of respiratory syncytial virus infections among children with acute respiratory symptoms in a community over three seasons
Molecular epidemiology of respiratory syncytial virus infections among children with acute respiratory symptoms in a community over three seasons
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DOI：
10.1128/jcm.43.1.36-40.2005
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发表时间：
2005-01-01
影响因子：
9.4
通讯作者：
Suzuki, H
中科院分区：
文献类型：
--
作者：
Sato, M;Saito, R;Suzuki, H
为了研究呼吸道合胞病毒（RSV）在社区中的分子流行病学，对2001年11月至2004年7月在日本新泻儿科门诊就诊的急性呼吸道症状儿童进行了三个季节的分析。在499例鼻咽抽吸物标本中，185例（37.1%）为RSV阳性，177例患者中只有8例（4.5%）经逆转录（RT）-PCR方法显示为再感染。RSV感染开始于初冬，春季感染率下降。在三个季节中，优势亚群从A变为B，然后又回到A。系统发育分析还显示，多个基因型共循环，每年，与基因型GA 5的亚组A占主导地位，在2001 - 2002年和2003-2004年的季节。在2002-2003年流行季节，在附着糖蛋白的第二个可变区插入60个核苷酸的亚组B（根据病毒命名法命名为BA）的新基因型作为新出现的毒株占主导地位，但这与其他季节由其他基因型引起的疾病病例不同，与新的流行病学或临床特征无关。总之，我们对RSV的分子分析证实，多种基因型每年共循环，占主导地位的基因型可能随季节而变化。支持通过RT-PCR确定基因型，作为表征社区RSV循环模式的有效工具。
To study the molecular epidemiology of respiratory syncytial virus (RSV) in a community, children with acute respiratory symptoms at a pediatric outpatient clinic in Niigata, Japan, were analyzed over three seasons from November 2001 to July 2004. Of 499 nasopharyngeal aspirate specimens, 185 (37.1%) were RSV positive, and only 8 (4.5%) of 177 patients were shown by the reverse transcription (RT)-PCR method to be reinfected. RSV infection occurred beginning in the early winter, and the rates declined in the spring. The predominant subgroup changed from A to B and returned to A over the three seasons. Phylogenetic analysis also revealed that multiple genotypes cocirculated each year, with genotype GA5 of subgroup A predominating in the 20012002 and the 2003-2004 seasons. A new genotype of subgroup B (named BA, according to the nomenclature for viruses) with a 60-nucleotide insertion in the second variable region of the attachment glycoportein protein was predominant as an emerging strain in the 2002-2003 season, but this was not associated with new epidemiological or clinical features, unlike the cases of disease caused by other genotypes in the other seasons. In conclusion, our molecular analysis of RSV confirms that multiple genotypes cocirculate each year and that the genotype predominating may shift with the season. Support for determination of the genotype by RT-PCR as an effective tool for characterization of RSV circulation patterns in the community is provided.