Luteolin exerted less inhibitory effect on macrophage activation induced by Astragalus polysaccharide than by lipopolysaccharide
Luteolin exerted less inhibitory effect on macrophage activation induced by Astragalus polysaccharide than by lipopolysaccharide
复制标题
木犀草素对黄芪多糖诱导的巨噬细胞活化的抑制作用弱于脂多糖
DOI：
10.1016/j.jff.2017.08.023
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发表时间：
2017-10-01
影响因子：
5.6
通讯作者：
Han, Quan-Bin
中科院分区：
文献类型：
--
作者：
Li, Zhi-Peng;Zhang, Quan-Wei;Han, Quan-Bin
作为一种食品补充，富含多糖的滋补草药越来越受到人们的欢迎。然而，关于已知活性分子与这些多糖之间的相互作用知之甚少。本研究旨在探讨木犀草素对黄芪多糖RAP诱导的RAW264.7细胞的影响，并与脂多糖（LPS）进行比较。结果显示木犀草素对lps诱导的RAW264.7细胞具有抑制炎症介质的作用。而在rap诱导的RAW264.7细胞中，木犀草素仅降低NO的产生和iNOS基因的表达，但仅在高浓度下抑制IL-6，对tnf - α无效。western blot分析结果显示，木樨草素在rap诱导的RAW264.7细胞中仅抑制NF-kappa B p65和Akt的磷酸化，而不抑制MAPKs和1RF3的磷酸化。综上所述，鲁特恩对RAP诱导的巨噬细胞的抑制作用小于LPS。本研究表明，含木犀草素的食物可能不会强烈干扰多糖RAP的免疫调节作用。(C) 2017 Elsevier Ltd.版权所有。
As a kind of food supplementary, polysaccharide-rich tonic herbs become more and more popular. However, little is known regarding the interaction between known active molecules with these polysaccharides. This study aims to investigate effects of luteolin on RAW264.7 cells induced by Astragalus polysaccharide RAP in comparison with lipopolysaccharide (LPS). The results showed that luteolin suppressed inflammatory meditors in LPS-induced RAW264.7 cells. While in the RAP-induced RAW264.7 cells, luteolin only decreased the NO production and iNOS gene expression, but only inhibit IL-6 at high concentration, and was ineffective in term of TNF-alpha. The results of western blot analysis suggested that luteolin only inhibited the phosphorylation of NF-kappa B p65 and Akt, but not MAPKs and 1RF3 in RAP-induced RAW264.7 cells. Taken together, lutelion exerted less inhibitory effect on macrophage induced by RAP than by LPS. This study demonstrated the luteolin-containing foods may not strongly interfere the immunoregulation effect of polysaccharide RAP. (C) 2017 Elsevier Ltd. All rights reserved.