Glycosphingolipids of normal bovine and enzootic bovine leukosis lymph node cells.
Glycosphingolipids of normal bovine and enzootic bovine leukosis lymph node cells.
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正常牛和地方性牛白血病淋巴结细胞的鞘糖脂。
DOI：
10.1093/oxfordjournals.jbchem.a121782
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发表时间：
1986
影响因子：
2.7
通讯作者：
S. Handa
中科院分区：
文献类型：
--
作者：
S. Watarai;T. Yasuda;M. Onuma;S. Handa
我们分析了7头牛的正常淋巴结细胞和8头患有地方性牛白血病的牛的淋巴结细胞中的鞘糖脂。中性鞘糖脂和神经节苷脂用薄层色谱法分析。正常和肿瘤淋巴结细胞都有GlcCer、LacCer和GbOse 3Cer作为主要的中性鞘糖脂。在神经节苷脂组分中，GM 3是正常和肿瘤淋巴结细胞中的主要组分，而另一组分神经节苷脂Gx组分在肿瘤淋巴结细胞中也很突出。该神经节苷脂Gx馏分的结构通过薄层色谱法、糖分析、神经氨酸酶消化和全甲基化研究来阐明。发现该神经节苷脂Gx级分是四种神经节苷脂种类的混合物。单个神经节苷脂Gx（1至4）的结构表征如下。1：GD3，NeuAc α 2-8NeuAc α 2- 3Gal 1 -4Glc-Cer。2：GD3，NeuAc α 2-8NeuGc α 2- 3Gal 1 -4Glc-Cer。3：GD3，NeuGc α 2-8NeuAc α 2- 3Gal 1-Glc-Cer。4：GD3，NeuGc α 2-8NeuGc α 2- 3Gal 1 -4Glc-Cer。这些GD 3物种可能是由于与恶性转化无关的诱导合成而形成的。
We analyzed glycosphingolipids from normal lymph node cells of seven cattle and lymph node cells of eight cattle with enzootic bovine leukosis. The neutral glycosphingolipids and gangliosides were analyzed by thin-layer chromatography. Both normal and tumorous lymph node cells had GlcCer, LacCer, and GbOse3Cer as major neutral glycosphingolipids. In the ganglioside fraction, GM3 was the predominant component in both normal and tumorous lymph node cells, and another component, ganglioside Gx fraction, was also prominent in tumorous lymph node cells. The structure of this ganglioside Gx fraction was elucidated by thin-layer chromatography, sugar analysis, neuraminidase digestion, and permethylation studies. This ganglioside Gx fraction was found to be a mixture of four ganglioside species. The structures of individual gangliosides Gx (1 to 4) were characterized as follows. 1: GD3, NeuAc alpha 2-8NeuAc alpha 2-3Gal1-4Glc-Cer. 2: GD3, NeuAc alpha 2-8NeuGc alpha 2-3Gal1-4Glc-Cer. 3: GD3, NeuGc alpha 2-8NeuAc alpha 2-3Gal1-Glc-Cer. 4: GD3, NeuGc alpha 2-8NeuGc alpha 2-3Gal1-4Glc-Cer. These GD3 species may be formed as a result of the induced synthesis inassociation with malignant transformation.