A novel method to study cerebrospinal fluid dynamics in rats.
A novel method to study cerebrospinal fluid dynamics in rats.
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DOI：
10.1016/j.jneumeth.2014.12.015
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发表时间：
2015-02-15
影响因子：
3
通讯作者：
Simard JM
中科院分区：
文献类型：
--
作者：
Karimy JK;Kahle KT;Kurland DB;Yu E;Gerzanich V;Simard JM
脑脊液（CSF）流动动力学在未成熟和成年大脑中起着关键作用，对神经发育和疾病过程（如脑积水和神经退行性变）具有重要意义。值得注意的是，迄今为止唯一报道的用于测量实验室大鼠中CSF形成的方法是间接示踪剂稀释法（a.k.a.，脑室脑池灌注），其具有局限性。将麻醉的大鼠固定在立体定位装置中，将两侧脑室插管，并闭塞侧脑室导水管。液体以等于CSF形成速率加上输注速率（如有）的速率流出一个脑室进入对侧脑室。以恒定的已知速率输注到对侧脑室中的药理学试剂被实时测试其对CSF形成的影响。测量的CSF形成率通过阻断侧脑室导水管而增加，但通过增加流出压力（0-3 cm H2O）而不改变。在雄性Wistar大鼠中，CSF形成具有年龄依赖性：8、9、10和12周时分别为0.39±0.06、0.74±0.05、1.02±0.04和1.40±0.06 µL/min。脑室内输注碳酸酐酶抑制剂乙酰唑胺可使CSF形成减少57%。示踪剂稀释法不允许持续实时测定CSF形成速率，不容易接受药理学操作，并且需要关键假设。CSF形成的直接测量克服了这些限制。直接测量大鼠CSF形成是可行的。我们的方法应该证明是有用的研究CSF动力学在正常的生理和疾病模型。
Cerebrospinal fluid (CSF) flow dynamics play critical roles in both the immature and adult brain, with implications for neurodevelopment and disease processes such as hydrocephalus and neurodegeneration. Remarkably, the only reported method to date for measuring CSF formation in laboratory rats is the indirect tracer dilution method (a.k.a., ventriculocisternal perfusion), which has limitations. Anesthetized rats were mounted in a stereotaxic apparatus, both lateral ventricles were cannulated, and the Sylvian aqueduct was occluded. Fluid exited one ventricle at a rate equal to the rate of CSF formation plus the rate of infusion (if any) into the contralateral ventricle. Pharmacological agents infused at a constant known rate into the contralateral ventricle were tested for their effect on CSF formation in real-time. The measured rate of CSF formation was increased by blockade of the Sylvian aqueduct but was not changed by increasing the outflow pressure (0–3 cm of H2O). In male Wistar rats, CSF formation was age-dependent: 0.39±0.06, 0.74±0.05, 1.02±0.04 and 1.40±0.06 µL/min at 8, 9, 10 and 12 weeks, respectively. CSF formation was reduced 57% by intraventricular infusion of the carbonic anhydrase inhibitor, acetazolamide. Tracer dilution methods do not permit ongoing real-time determination of the rate of CSF formation, are not readily amenable to pharmacological manipulations, and require critical assumptions. Direct measurement of CSF formation overcomes these limitations. Direct measurement of CSF formation in rats is feasible. Our method should prove useful for studying CSF dynamics in normal physiology and disease models.
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影响因子：
12.7
作者：
Arbel-Ornath M;Hudry E;Eikermann-Haerter K;Hou S;Gregory JL;Zhao L;Betensky RA;Frosch MP;Greenberg SM;Bacskai BJ
通讯作者：
Bacskai BJ
影响因子：
4
作者：
Christensen HL;Nguyen AT;Pedersen FD;Damkier HH
通讯作者：
Damkier HH
影响因子：
7.3
作者：
Baehr, Carsten;Reichel, Valeska;Fricker, Gert
通讯作者：
Fricker, Gert
影响因子：
17.1
作者：
Iliff JJ;Wang M;Liao Y;Plogg BA;Peng W;Gundersen GA;Benveniste H;Vates GE;Deane R;Goldman SA;Nagelhus EA;Nedergaard M
通讯作者：
Nedergaard M
影响因子：
7.3
作者：
Chiu C;Miller MC;Caralopoulos IN;Worden MS;Brinker T;Gordon ZN;Johanson CE;Silverberg GD
通讯作者：
Silverberg GD