A serological assay to detect SARS-CoV-2 antibodies in at-home collected finger-prick dried blood spots
A serological assay to detect SARS-CoV-2 antibodies in at-home collected finger-prick dried blood spots
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DOI：
10.1101/2020.05.29.20116004
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发表时间：
2020-11-19
影响因子：
4.6
通讯作者：
Tsai, Cheng-ting
中科院分区：
文献类型：
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作者：
Karp, Donna Grace;Danh, Kenneth;Tsai, Cheng-ting
准确监测2019冠状病毒病（COVID-19）的发病率需要对人群进行大规模检测。目前的检测方法需要由医护人员亲自采集生物样本，限制了无法接触检测设施的个人的接触，同时使患者和医护人员都面临感染风险。我们报告的开发和验证的家庭手指刺干血斑采集试剂盒和分析方法。我们证明了100%的灵敏度和特异性使用在美国各地的家庭收集的标本。这种方法可以促进在难以接触的人群中进行无偏见的血清调查，并有助于减少卫生保健系统和个人的血清学测试的样品收集负担。
Accurate surveillance of coronavirus disease 2019 (COVID-19) incidence requires large-scale testing of the population. Current testing methods require in-person collection of biospecimens by a healthcare worker, limiting access of individuals who do not have access to testing facilities while placing both patients and healthcare workers at risk of exposure to infection. We report the development and validation of a at-home finger-prick dried blood spot collection kit and an analysis method. We demonstrated 100% sensitivity and specificity using at-home collected specimens across the US. Such methods may facilitate the conduct of unbiased serosurveys within hard to reach populations and help reduce the sample collection burden of serological testing on both health care systems and individuals alike.